Department of Chemical and Physical Sciences, Faculty of Natural Sciences, Walter Sisulu University, Mthatha 5099, South Africa.
Department of Pharmacognosy, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife 220005, Nigeria.
Molecules. 2021 Oct 28;26(21):6528. doi: 10.3390/molecules26216528.
is a hemi-parasitic plant used in African ethnomedicine for the management of microbial infections, rheumatic pain and tumors amongst others. We report the isolation and characterization of eight compounds with their antioxidant and antimicrobial activities. The air-dried powdered leaf was macerated in EtOH/H0 (4:1). The extract was solvent-partitioned into -hexane, EtOAc, -BuOH and aqueous fractions. The fractions were screened for their antioxidant properties, using DPPH, FRAP, TAC and FIC assays. Antimicrobial analysis was performed using the micro-broth dilution method. The active EtOAc fraction was purified for its putative compounds on a repeated silica gel column chromatography monitored with TLC-bioautography. The isolated compounds were characterized using spectroscopic methods of UV, FT-IR, NMR and MS. Eight compounds (-) were isolated and characterized as 13,27-cycloursane (), phyllanthone (), globraunone (), three phenolics: methyl 3,5-dihydroxy-4-methoxybenzoate (), methyl 3-methyl-4-hydroxybenzoate () and guaiacol (), as well as two phenol derivatives: 4-formaldehyde phenone () and 6-methoxy--inden-5-ol (). The study identified and as natural antioxidant compounds with potential as antimicrobial agents.
是一种半寄生植物，在非洲传统医学中用于治疗微生物感染、风湿痛和肿瘤等疾病。我们报告了八种化合物的分离和鉴定及其抗氧化和抗菌活性。将风干的粉状叶在 EtOH/H0（4:1）中浸渍。提取物用正己烷、EtOAc、-BuOH 和水相进行溶剂分离。使用 DPPH、FRAP、TAC 和 FIC 测定法筛选各馏分的抗氧化性能。使用微量肉汤稀释法进行抗菌分析。用硅胶柱色谱法对具有抗氧化活性的 EtOAc 馏分进行分离，并用 TLC-生物自显影法进行监测。用紫外光谱、傅里叶变换红外光谱、核磁共振和质谱等光谱方法对分离得到的化合物进行了表征。分离得到了八种化合物，分别鉴定为 13,27-环羊毛甾烷（）、叶素（）、globraunone（）、三种酚类化合物：3,5-二羟基-4-甲氧基苯甲酸甲酯（）、3-甲基-4-羟基苯甲酸甲酯（）和愈创木酚（），以及两种酚类衍生物：4-甲醛苯酮（）和 6-甲氧基--茚-5-醇（）。该研究鉴定了 和 为天然抗氧化化合物，具有作为抗菌剂的潜力。
