Robak T, Korycka A
Arch Immunol Ther Exp (Warsz). 1987;35(1):33-42.
The antigenic characteristics of the leukaemic cell population in 31 patients with acute myeloid leukaemia (AML) and 5 patients with acute undifferentiated leukaemia (AUL) was investigated using a panel of 15 monoclonal antibodies (Mc Abs). We chose 14 Mc Abs that react with lineage--and stage related myeloid antigens and L243 Mc Ab that reacts with HLA-DR antigen. In AML cases we correlated the antigenic phenotype with morphological FAB classification. The study indicates a substantial antigenic heterogeneity of the surface antigen expression on leukaemia cells particularly in M1, M2 and M4 AML cases. The morphological subtype of these leukaemias tended to correlate with the immunologic phenotype, particularly in more differentiated AML cases such as M3 or M5. The most immature cell phenotype characterised "undifferentiated" AML, which was expressed by the reactivity or L243, BI3C5, MY9, VIM-2 and S3-13 Mc Abs with the majority of the patients. The analysis shows that although there is a tendency for the morphology to correlate with the surface antigen phenotype each morphological group contains patients having different surface antigen phenotype.
运用一组15种单克隆抗体（Mc Abs）对31例急性髓细胞白血病（AML）患者及5例急性未分化白血病（AUL）患者白血病细胞群体的抗原特性进行了研究。我们选取了14种与谱系及阶段相关髓系抗原发生反应的单克隆抗体以及与HLA - DR抗原发生反应的L243单克隆抗体。在AML病例中，我们将抗原表型与形态学FAB分类进行了关联分析。研究表明，白血病细胞表面抗原表达存在显著的抗原异质性，尤其在M1、M2和M4 AML病例中。这些白血病的形态学亚型往往与免疫表型相关，特别是在分化程度较高的AML病例如M3或M5中。最不成熟的细胞表型特征为“未分化”AML，大多数患者通过L243、BI3C5、MY9、VIM - 2和S3 - 13单克隆抗体的反应性表现出来。分析表明，尽管形态学有与表面抗原表型相关的趋势，但每个形态学组中都包含具有不同表面抗原表型的患者。
