Borszéková Pulzová Lucia, Roška Jan, Kalman Michal, Kliment Ján, Slávik Pavol, Smolková Božena, Goffa Eduard, Jurkovičová Dana, Kulcsár Ľudovít, Lešková Katarína, Bujdák Peter, Mego Michal, Bhide Mangesh R, Plank Lukáš, Chovanec Miroslav
Biomedical Research Center, Department of Genetics, Cancer Research Institute, Slovak Academy of Sciences, Dúbravská cesta 9, 845 05 Bratislava, Slovakia.
Department of Pathological Anatomy, Jessenius Faculty of Medicine and University Hospital in Martin, Comenius University, Malá Hora 4A, 036 01 Martin, Slovakia.
Cancers (Basel). 2021 Nov 8;13(21):5573. doi: 10.3390/cancers13215573.
Rete testis invasion (RTI) is an unfavourable prognostic factor for the risk of relapse in clinical stage I (CS I) seminoma patients. Notably, no evidence of difference in the proteome of RTI-positive vs. -negative CS I seminomas has been reported yet. Here, a quantitative proteomic approach was used to investigate RTI-associated proteins. 64 proteins were differentially expressed in RTI-positive compared to -negative CS I seminomas. Of them, 14-3-3γ, ezrin, filamin A, Parkinsonism-associated deglycase 7 (PARK7), vimentin and vinculin, were validated in CS I seminoma patient cohort. As shown by multivariate analysis controlling for clinical confounders, PARK7 and filamin A expression lowered the risk of RTI, while 14-3-3γ expression increased it. Therefore, we suggest that in real clinical biopsy specimens, the expression level of these proteins may reflect prognosis in CS I seminoma patients.
睾丸网浸润（RTI）是临床I期（CS I）精原细胞瘤患者复发风险的一个不良预后因素。值得注意的是，目前尚未有关于RTI阳性与阴性CS I精原细胞瘤蛋白质组差异的报道。在此，我们采用定量蛋白质组学方法来研究与RTI相关的蛋白质。与RTI阴性的CS I精原细胞瘤相比，有64种蛋白质在RTI阳性的肿瘤中差异表达。其中，14-3-3γ、埃兹蛋白、细丝蛋白A、帕金森病相关去糖基化酶7（PARK7）、波形蛋白和纽蛋白在CS I精原细胞瘤患者队列中得到了验证。多因素分析控制临床混杂因素显示，PARK7和细丝蛋白A的表达降低了RTI风险，而14-3-3γ的表达增加了RTI风险。因此，我们认为在实际临床活检标本中，这些蛋白质的表达水平可能反映CS I精原细胞瘤患者的预后。
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