miR-497-5p 通过下调 CDCA4 抑制肺鳞癌细胞的进展。
MiR-497-5p down-regulates CDCA4 to restrains lung squamous cell carcinoma progression.
机构信息
Department of Cardiovascular Surgery, Yiwu Central Hospital, No.699 Jiangdong Dong Lu, Yiwu City, 322000, Zhejiang Province, China.
出版信息
J Cardiothorac Surg. 2021 Nov 12;16(1):330. doi: 10.1186/s13019-021-01698-2.
BACKGROUND
So far, few have concerned miR-497-5p in lung squamous cell carcinoma (LUSC).
METHODS
MiR-497-5p expression in LUSC was measured by qRT-PCR. Its impacts on tumor-related cell behaviors were investigated by CCK8 assay, scratch healing assay, flow cytometry and Transwell invasion methods. In addition, interaction between miR-497-5p and CDCA4 in LUSC was also elucidated through rescue experiment, western blot, dual-luciferase, and bioinformatics analysis.
RESULTS
Low level of miR-497-5p was confirmed in LUSC tissue and cells. Overexpressed miR-497-5p markedly inhibited cancer progression. miR-497-5p restrained CDCA4 expression. Rescue assay showed that overexpressing miR-497-5p eliminated effect of overexpressed CDCA4.
CONCLUSION
By targeting CDCA4, miR-497-5p restrained development of LUSC.
背景
到目前为止，很少有人关注肺鳞癌（LUSC）中的 miR-497-5p。
方法
通过 qRT-PCR 检测 LUSC 中 miR-497-5p 的表达。通过 CCK8 检测、划痕愈合检测、流式细胞术和 Transwell 侵袭实验研究其对肿瘤相关细胞行为的影响。此外，还通过挽救实验、western blot、双荧光素酶报告基因和生物信息学分析阐明了 miR-497-5p 与 LUSC 中 CDCA4 之间的相互作用。
结果
在 LUSC 组织和细胞中证实 miR-497-5p 水平较低。过表达 miR-497-5p 可显著抑制癌症进展。miR-497-5p 抑制 CDCA4 的表达。挽救实验表明，过表达 miR-497-5p 消除了过表达 CDCA4 的作用。
结论
通过靶向 CDCA4，miR-497-5p 抑制了 LUSC 的发展。
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