Department of Biology, Lund University, Lund, Sweden.
Department of Plant Protection, King Saud University, Chair of Date Palm Research, Chemical Ecology and Functional Genomics Laboratory, College of Food and Agricultural Sciences, Riyadh, Saudi Arabia.
Pest Manag Sci. 2022 Mar;78(3):1048-1059. doi: 10.1002/ps.6716. Epub 2021 Dec 6.
(Z,E)-9,12-tetradecadienyl acetate (ZETA, Z9,E12-14:OAc) is a major sex pheromone component for many stored-product moth species. This pheromone is used worldwide for mating disruption, detection, monitoring, and mass trapping in raw and processed food storage facilities. In this study, we demonstrate the biological production of ZETA pheromone by engineered yeast Saccharomyces cerevisiae.
We mined the pheromone gland transcriptome data of the almond moth, Ephestia (Cadra) cautella (Walker), to trace a novel E12 fatty acyl desaturase and expressed candidates heterologously in yeast and Sf9 systems. Furthermore, we demonstrated a tailor-made ZETA pheromone bioproduction in yeast through metabolic engineering using this E12 desaturase, in combination with three genes from various sources coding for a Z9 desaturase, a fatty acyl reductase, and an acetyltransferase, respectively. Electrophysiological assays (gas chromatography coupled to an electroantennographic detector) proved that the transgenic yeast-produced ZETA pheromone component elicits distinct antennal responses.
The reconstructed biosynthetic pathway in yeast efficiently produces ZETA pheromone, leaves an undetectable level of biosynthetic intermediates, and paves the way for the economically competitive high-demand ZETA pheromone's bioproduction technology for high-value storage pest control.
（Z，E）-9,12-十四碳二烯基乙酸酯（ZETA，Z9，E12-14：OAc）是许多储存产品蛾类的主要性信息素成分。这种信息素被全世界用于交配干扰、检测、监测和大量诱捕原始和加工食品储存设施中的害虫。在这项研究中，我们证明了工程酵母酿酒酵母（Saccharomyces cerevisiae）生物合成 ZETA 信息素。
我们挖掘了杏仁蛾 Ephestia（Cadra） cautella（Walker）的信息素腺转录组数据，以追踪一种新型的 E12 脂肪酸去饱和酶，并在酵母和 Sf9 系统中异源表达候选物。此外，我们通过使用这种 E12 去饱和酶的代谢工程，结合来自不同来源的三个基因，分别编码 Z9 去饱和酶、脂肪酸还原酶和乙酰基转移酶，在酵母中展示了定制的 ZETA 信息素生物生产。电生理学测定（气相色谱与电触角探测耦合）证明，转基因酵母产生的 ZETA 信息素成分引起了明显的触角反应。
酵母中重建的生物合成途径有效地产生了 ZETA 信息素，留下了无法检测到的生物合成中间体水平，并为高价值储存害虫控制的高需求 ZETA 信息素的经济上有竞争力的生物生产技术铺平了道路。
