Jahanmard Elham, Keramat Javad, Nasirpour Ali, Emadi Rahmatollah
Department of Food Sciences and Technology, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
Department of Materials Engineering, Isfahan University of Technology, Isfahan, 84156-83111, Iran.
J Food Sci. 2021 Dec;86(12):5200-5212. doi: 10.1111/1750-3841.15946. Epub 2021 Nov 13.
Detoxification of aflatoxin M from solution and milk using layered double hydroxides was investigated. The Aluminum-Magnesium layered double hydroxide (Al-Mg LDH) and Iron-Magnesium layered double hydroxide (Fe-Mg LDH) were selected in their calcined and non-calcined forms to evaluate the effect of the calcination on detoxification. These materials were produced using the co-precipitation method. Preliminary adsorption tests confirmed use of Al-Mg LDH as the selected adsorbent. Characteristics of the adopted adsorbent were studied and confirmed by XRD, FTIR, SEM, and BET methods. Effects of the initial content of aflatoxin, amount of the adsorbents and detoxification time were investigated. Influence of the adsorbents on the nutritional aspects of milk were also studied. The study showed that while the non-calcined forms of LDH were not able to adsorb aflatoxin M more than 23%, the calcined form of Al-Mg LDH exhibited 100% adsorption in the solutions and about 70-100% in the contaminated milk samples. The reason is pointed to the fact that calcination of Al-Mg LDH considerably increased the surface area, the total pore volume, and the pore size of the material. Multivariate regression analysis and calculation of the Pearson correlation factor showed that the remained aflatoxin at each time was more strongly correlated with the initial amount of aflatoxin and the elapsed time and less strongly with the amount of the adsorbent. It was found that the adsorption isotherms fitted to the Freundlich equation with a high adsorption capacity of 555.5 mg g-1. PRACTICAL APPLICATION: This study is focused on examining ability of layered double hydroxides (LDH) for adsorbing AFM . LDHs are promising layered materials due to some of their interesting characteristics, such as ease of synthesis and uniqueness of structure. In practice, results of this study can be used for detoxification of aflatoxin, especially in milk, at high efficiency in shorter time durations.
研究了使用层状双氢氧化物从溶液和牛奶中去除黄曲霉毒素M的方法。选用了煅烧和未煅烧形式的铝镁层状双氢氧化物（Al-Mg LDH）和铁镁层状双氢氧化物（Fe-Mg LDH）来评估煅烧对解毒效果的影响。这些材料采用共沉淀法制备。初步吸附试验证实选用Al-Mg LDH作为吸附剂。通过XRD、FTIR、SEM和BET方法对所采用吸附剂的特性进行了研究和确认。研究了黄曲霉毒素初始含量、吸附剂用量和解毒时间的影响。还研究了吸附剂对牛奶营养成分的影响。研究表明，虽然未煅烧形式的LDH对黄曲霉毒素M的吸附率不超过23%，但煅烧形式的Al-Mg LDH在溶液中表现出100%的吸附率，在受污染的牛奶样品中表现出约70-100%的吸附率。原因是Al-Mg LDH的煅烧显著增加了材料的表面积、总孔体积和孔径。多元回归分析和皮尔逊相关系数计算表明，每次剩余的黄曲霉毒素与黄曲霉毒素的初始量和经过的时间相关性更强，与吸附剂用量的相关性较弱。发现吸附等温线符合Freundlich方程，吸附容量高达555.5 mg g-1。实际应用：本研究重点考察层状双氢氧化物（LDH）吸附AFM的能力。LDH因其一些有趣的特性，如易于合成和结构独特性，是很有前景的层状材料。在实际中，本研究结果可用于黄曲霉毒素的解毒，特别是在牛奶中，能在较短时间内高效解毒。
