Li S D, Zhang L R, Luo X, Ao P
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
Zhonghua Zhong Liu Za Zhi. 1987 Mar;9(2):90-4.
The cytogenetic studies on three esophageal cancer cell lines established in China were done. Thirty metaphases showing suitable chromosome length and good G-banding pattern from each of the cell lines were chosen and subjected to karyological analysis. The typical karyotypes from these cell lines were listed, and the variation of chromosome number as well as the morphological characteristics, possible sources and the incidence of the marker chromosomes were analysed. Eca 109 is the cell line first established and used extensively in China. A strictly regular karyotype pattern was found in it: the modal number of chromosomes being 63-64; the number of each type of chromosome varying between 1-5; a distal deletion of short arm of chromosome No. 1 being discernible in all metaphases, with break sites located within 1p22-1p33. Also a distal deletion of long arm of chromosome No. 4 was usually visible. There were seven marker chromosomes with high incidence. Among them, M1 marker chromosome was a large subacrocentric chromosome which was observed in the early passages of this cell line. The chromosome number of Ec 17 cell line was usually subtetraploid. In addition to the numerical variation in some of the chromosomes, six marker chromosomes were usually observed. Among them, M1 involved reciprocal translocation between chromosome No. 1 and No. 4. M3 of Ec 17 was in correspondence with M5 of Eca 109. Both were Rob (13q;14q). The chromosome number of Ec 56 was usually subtetraploid, and in addition to the numerical variation in some of the chromosomes, seven marker chromosomes were usually observed.
对在中国建立的三株食管癌细胞系进行了细胞遗传学研究。从每个细胞系中选取30个中期分裂相，这些中期分裂相的染色体长度合适且G带模式良好，然后进行核型分析。列出了这些细胞系的典型核型，并分析了染色体数目的变异以及标记染色体的形态特征、可能来源和发生率。Eca 109是中国最早建立并广泛应用的细胞系。在该细胞系中发现了严格规则的核型模式：染色体众数为63 - 64；每种类型染色体的数目在1 - 5之间变化；在所有中期分裂相中均可观察到1号染色体短臂的远端缺失，断裂位点位于1p22 - 1p33内。此外，通常还可见到4号染色体长臂的远端缺失。有7条标记染色体发生率较高。其中，M1标记染色体是一条大的亚近端着丝粒染色体，在该细胞系的早期传代中即可观察到。Ec 17细胞系的染色体数目通常为亚四倍体。除了一些染色体的数目变异外，通常还观察到6条标记染色体。其中，M1涉及1号和4号染色体之间的相互易位。Ec 17的M3与Eca 109的M5相对应。两者均为罗伯逊易位（13q;14q）。Ec 56的染色体数目通常为亚四倍体，除了一些染色体的数目变异外，通常还观察到7条标记染色体。
