傅里叶变换红外光谱法研究合成卵清蛋白纳米粒子。
FTIR study of synthesized ovalbumin nanoparticles.
机构信息
Department of Physics, Annamalai University, Chidambaram, 608 002, TN, India.
Department of Physics, Annamalai University, Chidambaram, 608 002, TN, India.
出版信息
Anal Biochem. 2022 Jan 1;636:114456. doi: 10.1016/j.ab.2021.114456. Epub 2021 Nov 11.
Ovalbumin particles are reduced to nano size using heat treatment techniques. Their structural patterns in their native state and in their pH denatured state were attempted. Denaturation is also a part of conformation and hence conformations due to pH and glucose were analyzed using FTIR spectroscopy. The interactions behind these conformations are unraveled and the role of glucose as cosolvent in restricting the denaturation is also revealed from the observed secondary structures of ovalbumin. Further, the characterization of these synthesized nano particles reveals the extent of their applications. The obtained results indicate that consideration of ovalbumin nanoparticles seems to favor a very clear trend of protein denaturation and the observed structural modifications are the result of development of non-covalent interactions by the cosolvent molecules.
卵清蛋白颗粒通过热处理技术被还原为纳米尺寸。尝试了它们在天然状态和 pH 变性状态下的结构模式。变性也是构象的一部分，因此使用傅里叶变换红外光谱法分析了由于 pH 和葡萄糖引起的构象。揭示了这些构象背后的相互作用，并且从卵清蛋白观察到的二级结构中也揭示了葡萄糖作为共溶剂在限制变性中的作用。此外，这些合成纳米颗粒的特性揭示了它们的应用程度。所得结果表明，考虑卵清蛋白纳米颗粒似乎有利于非常明显的蛋白质变性趋势，并且观察到的结构修饰是由共溶剂分子形成非共价相互作用的结果。
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