Wöhner Thomas W, Emeriewen Ofere F, Wittenberg Alexander H J, Schneiders Harrie, Vrijenhoek Ilse, Halász Júlia, Hrotkó Károly, Hoff Katharina J, Gabriel Lars, Lempe Janne, Keilwagen Jens, Berner Thomas, Schuster Mirko, Peil Andreas, Wünsche Jens, Kropop Stephan, Flachowsky Henryk
Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Pillnitzer Platz 3a, D-01326, Dresden, Germany.
Julius Kühn Institute (JKI) - Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Pillnitzer Platz 3a, D-01326, Dresden, Germany.
Genomics. 2021 Nov;113(6):4173-4183. doi: 10.1016/j.ygeno.2021.11.002. Epub 2021 Nov 11.
Cherries are stone fruits and belong to the economically important plant family of Rosaceae with worldwide cultivation of different species. The ground cherry, Prunus fruticosa Pall., is an ancestor of cultivated sour cherry, an important tetraploid cherry species. Here, we present a long read chromosome-level draft genome assembly and related plastid sequences using the Oxford Nanopore Technology PromethION platform and R10.3 pore type. We generated a final consensus genome sequence of 366 Mb comprising eight chromosomes. The N50 scaffold was ~44 Mb with the longest chromosome being 66.5 Mb. The chloroplast and mitochondrial genomes were 158,217 bp and 383,281 bp long, which is in accordance with previously published plastid sequences. This is the first report of the genome of ground cherry (P. fruticosa) sequenced by long read technology only. The datasets obtained from this study provide a foundation for future breeding, molecular and evolutionary analysis in Prunus studies.
樱桃是核果类水果,属于经济上重要的蔷薇科植物家族,该家族的不同物种在全球范围内均有种植。欧洲酸樱桃(Prunus fruticosa Pall.)是栽培酸樱桃的祖先,栽培酸樱桃是一种重要的四倍体樱桃品种。在此,我们使用牛津纳米孔技术PromethION平台和R10.3孔型,展示了一个长读长染色体水平的基因组草图组装及相关的质体序列。我们生成了一个最终的共有基因组序列,大小为366 Mb,包含八条染色体。N50支架约为44 Mb,最长的染色体为66.5 Mb。叶绿体和线粒体基因组长度分别为158,217 bp和383,281 bp,这与先前发表的质体序列一致。这是仅通过长读长技术对欧洲酸樱桃(P. fruticosa)基因组进行测序的首次报道。本研究获得的数据集为李属研究中的未来育种、分子和进化分析奠定了基础。