SaBio. Instituto de Investigación en Recursos Cinegéticos IREC (CSIC-UCLM-JCCM), Ronda de Toledo 12, Ciudad Real 13005, Spain.
Medianilla S.A., Benalup-Casas Viejas, Spain.
Ticks Tick Borne Dis. 2022 Jan;13(1):101864. doi: 10.1016/j.ttbdis.2021.101864. Epub 2021 Nov 3.
The genus Anaplasma contains various species capable of causing disease in animals and humans. Anaplasma marginale is one of the main tick-borne pathogens of bovines in tropical and subtropical regions; however, these bacteria are now being detected more frequently in other regions of the world including Europe. In July 2017, abortions, mortality and morbidity in Retinta breed of cattle were investigated in southwestern Spain. Based on clinical signs, the provisional clinical diagnosis of bovine anaplasmosis was made. A molecular-phylogenetic approach was used to characterize A. marginale using multiple markers, including 16S rRNA, msp1a, msp4 and msp5 genes. The msp1α sequence was different from the previously described sequences from Spain as well as other countries. The isolates of A. marginale were classified as Genotype C, with two of the five tandem repeats in the amino acid sequences MSP1α being novel. The highest variability was observed in the four sequences of msp5 which was depicted in their clustering into multiple clades on a phylogenetic tree. Comparison of msp5 nucleotide sequences and the corresponding amino acid sequences revealed the co-existence of different strains in the same region. This study highlights the occurrence of clinical bovine anaplasmosis in an endemic region of Spain.
无形体属包含多种能够引起动物和人类疾病的物种。边缘无形体是热带和亚热带地区牛的主要蜱传病原体之一；然而，这些细菌现在在世界其他地区（包括欧洲）越来越频繁地被检测到。2017 年 7 月，在西班牙西南部调查了 Retinta 品种牛的流产、死亡率和发病率。根据临床症状，做出了牛无形体病的临时临床诊断。使用多种标记物，包括 16S rRNA、msp1a、msp4 和 msp5 基因，采用分子系统发育方法对边缘无形体进行了特征描述。msp1α 序列与西班牙和其他国家以前描述的序列不同。分离的边缘无形体被分类为基因型 C，在 MSP1α 的 5 个串联重复氨基酸序列中有 2 个是新的。msp5 的四个序列观察到最高的变异性，在系统发育树上它们聚类为多个分支。msp5 核苷酸序列和相应氨基酸序列的比较表明，在同一地区存在不同的菌株共存。本研究强调了西班牙地方性流行地区临床牛无形体病的发生。
