弗氏红白血病细胞中 APRT 基因座的突变。1. 突变率及突变体特性。

Mutation at the APRT locus in Friend erythroleukaemia cells. 1. Mutation rates and properties of mutants.

作者信息

McKenna P G, Ward P E

机构信息

Biomedical Sciences Research Centre, University of Ulster, Coleraine, Northern Ireland.

出版信息

Mutat Res. 1987 Oct;180(2):267-71. doi: 10.1016/0027-5107(87)90223-5.

DOI:10.1016/0027-5107(87)90223-5

PMID:3477692

Abstract

Spontaneous mutation at the adenine phosphoribosyl transferase (APRT) locus in clone 707 of the Friend cell line was examined. The frequency of cells resistant to 2,6-diaminopurine (DAP) was found to be 2.6 X 10(-5) with a mutation rate of 1.81 X 10(-6) cell-1 generation-1. APRT activities in 9 DAP-resistant clones were found to vary between 0 and 27% the level observed in wild-type cells. It is suggested that clone 707 cells are heterozygous or functionally hemizygous at the APRT locus.

摘要

对弗瑞德细胞系707克隆株腺嘌呤磷酸核糖转移酶（APRT）位点的自发突变进行了检测。发现对2,6 - 二氨基嘌呤（DAP）耐药的细胞频率为2.6×10⁻⁵，突变率为1.81×10⁻⁶细胞⁻¹代⁻¹。在9个DAP耐药克隆株中，APRT活性在野生型细胞中观察到的水平的0%至27%之间变化。提示707克隆株细胞在APRT位点是杂合的或功能上半合子的。