Reis Mateus Neri Oliveira, Bessa Layara Alexandre, de Jesus Andressa Pereira, Guimarães Silva Fabiano, Moreira Marialva Alvarenga, Vitorino Luciana Cristina
Laboratory of Agricultural Microbiology, Instituto Federal Goiano - Rio Verde Campus, Highway Sul Goiana, Rio Verde, Brazil.
Laboratory of Plant Mineral Nutrition and CEAGRE - Exponential Agriculture Center of Excellence, Instituto Federal Goiano, Rio Verde, Brazil.
Front Plant Sci. 2021 Oct 29;12:759463. doi: 10.3389/fpls.2021.759463. eCollection 2021.
The sustainable development of agriculture can be stimulated by the great market availability of bio-inputs, including phosphate-solubilizing microbial strains. However, these strains are currently selected using imprecise and questionable solubilization methodologies in solid or liquid media. We hypothesized that the hydroponic system could be a more efficient methodology for selecting phosphate-solubilizing strains as plant growth promoters. This methodology was tested using the plant as a model. The growth-promoting potential of the strains was compared with that of the Biomaphos® commercial microbial mixture. The obtained calcium phosphate (CaHPO) solubilization results using the hydroponic system were inconsistent with those observed in solid and liquid media. However, the tests in liquid medium demonstrated poor performances of sp. (328EF) and (33EF) in reducing pH and solubilizing CaHPO, which corroborates with the effects of biotic stress observed in plants inoculated with these strains. Nevertheless, the hydroponic system allowed the characterization of (PA12), which is also efficient in solubilization in a liquid medium. The bacterium (PA26) was the most effective in CaHPO solubilization owing to the higher phosphorus (P) absorption, growth promotion, and physiological performance observed in plants inoculated with this bacterium. The hydroponic method proved to be superior in selecting solubilizing strains, allowing the assessment of multiple patterns, such as nutritional level, growth, photosynthetic performance, and anatomical variation in plants, and even the detection of biotic stress responses to inoculation, obtaining strains with higher growth promotion potential than Biomaphos®. This study proposed a new approach to confirm the solubilizing activity of microorganisms previously selected and potentially intended for the bio-input market that are useful in P availability for important crops, such as soybeans.
包括解磷微生物菌株在内的生物投入品在市场上的大量供应，能够促进农业的可持续发展。然而，目前这些菌株是在固体或液体培养基中使用不精确且有问题的溶解方法筛选出来的。我们推测水培系统可能是一种更有效的筛选作为植物生长促进剂的解磷菌株的方法。以植物为模型对该方法进行了测试。将这些菌株的促生长潜力与Biomaphos®商业微生物混合物的促生长潜力进行了比较。使用水培系统获得的磷酸钙（CaHPO）溶解结果与在固体和液体培养基中观察到的结果不一致。然而，在液体培养基中的测试表明，sp.（328EF）和（33EF）在降低pH值和溶解CaHPO方面表现不佳，这与接种这些菌株的植物中观察到的生物胁迫效应相符。尽管如此，水培系统能够对（PA12）进行特性描述，该菌株在液体培养基中也具有高效的溶解能力。由于接种该细菌的植物中观察到更高的磷（P）吸收、生长促进和生理性能，细菌（PA26）在CaHPO溶解方面最为有效。事实证明，水培方法在筛选溶解菌株方面更具优势，它可以评估多种模式，如植物的营养水平、生长、光合性能和解剖变异，甚至检测接种后的生物胁迫反应，从而获得比Biomaphos®具有更高促生长潜力的菌株。本研究提出了一种新方法，以确认先前筛选出的、可能用于生物投入品市场的微生物的溶解活性，这些微生物对重要作物（如大豆）的磷有效性有用。
