Differential DNA methylation between long-winged and short-winged adults of .

a catastrophic rice pest in South East Asia, has adults with wing dimorphism. DNA methylation has been proved to play an important role in regulation of phenotype differentiation in insects. In this study, methylation sensitive amplification polymorphism (MSAP) was used to investigate the cytosine methylation state at CCGG sites in macropterous male adults (MMA) and brachypterous male adults (BMA) of brown planthopper. In MMA, the fully methylated ratio was 2.96%, hemi-methylated ratio 3.83% and total methylated ratio 6.79%. In BMA, they were 5.53%, 4.19% and 9.72%, respectively. There were significant differences in the methylation of the target sites (CCGG) between MMA and BMA (ØST = 0.2614,  = 0.0354). Based the PCoA results, a much clear separation were also shown between MMA and BMA along the first coordinate (38.8% of variance explained). We also cloned and got nine satisfactory sequences with different methylation states between MMA and BMA. Two of them have similarity with male-specific sequence in chromosome Y and lipophorin receptor gene in respectively. The result showed that the methylation patterns and levels were different between two wing phenotypes of and will facilitate research on the epigenetic mechanism of insect wing dimorphism.