Yang Mei, Liang Shike, Wang Fanghai
State Key Laboratory for Biocontrol and Institute of Entomology, Sun Yat-sen University, Guangzhou, 510275 China.
3 Biotech. 2021 Nov;11(11):476. doi: 10.1007/s13205-021-03026-w. Epub 2021 Oct 26.
a catastrophic rice pest in South East Asia, has adults with wing dimorphism. DNA methylation has been proved to play an important role in regulation of phenotype differentiation in insects. In this study, methylation sensitive amplification polymorphism (MSAP) was used to investigate the cytosine methylation state at CCGG sites in macropterous male adults (MMA) and brachypterous male adults (BMA) of brown planthopper. In MMA, the fully methylated ratio was 2.96%, hemi-methylated ratio 3.83% and total methylated ratio 6.79%. In BMA, they were 5.53%, 4.19% and 9.72%, respectively. There were significant differences in the methylation of the target sites (CCGG) between MMA and BMA (ØST = 0.2614, = 0.0354). Based the PCoA results, a much clear separation were also shown between MMA and BMA along the first coordinate (38.8% of variance explained). We also cloned and got nine satisfactory sequences with different methylation states between MMA and BMA. Two of them have similarity with male-specific sequence in chromosome Y and lipophorin receptor gene in respectively. The result showed that the methylation patterns and levels were different between two wing phenotypes of and will facilitate research on the epigenetic mechanism of insect wing dimorphism.
褐飞虱是东南亚一种具有灾难性危害的水稻害虫，其成虫具有翅二型性。DNA甲基化已被证明在昆虫表型分化调控中起重要作用。本研究采用甲基化敏感扩增多态性（MSAP）技术，研究了褐飞虱长翅型雄成虫（MMA）和短翅型雄成虫（BMA）中CCGG位点的胞嘧啶甲基化状态。在MMA中，全甲基化率为2.96%，半甲基化率为3.83%，总甲基化率为6.79%。在BMA中，它们分别为5.53%、4.19%和9.72%。MMA和BMA之间目标位点（CCGG）的甲基化存在显著差异（ØST = 0.2614， = 0.0354）。基于主坐标分析（PCoA）结果，在第一坐标上MMA和BMA之间也表现出明显的分离（解释了38.8%的方差）。我们还克隆并获得了9个在MMA和BMA之间具有不同甲基化状态的满意序列。其中两个分别与Y染色体上的雄性特异性序列和脂磷蛋白受体基因具有相似性。结果表明，褐飞虱两种翅型之间的甲基化模式和水平不同，这将有助于昆虫翅二型性表观遗传机制的研究。
