State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, State Key Laboratory Base of Novel Functional Materials and Preparation Science, School of Materials Science and Chemical Engineering, Ningbo University, Ningbo, People's Republic of China.
Luminescence. 2022 Feb;37(2):220-229. doi: 10.1002/bio.4163. Epub 2021 Nov 26.
In this paper, based on the fluorescence of carbon quantum dots (CQDs) quenched by mercury ions (Hg ) and the nonresponse of Hg to rhodamine B fluorescence, a dual emission ratio fluorescence sensor was constructed to realize the quantitative detection of Hg . Under excitation at 365 nm, the fluorescence spectrum showed double emission peaks at 437 nm and 590 nm, corresponding to the fluorescence emissions of CQDs and rhodamine B, respectively. This method quantitatively detected Hg based on the linear relationship between the ratio of the intensities of the two emission peaks F /F and the concentration of Hg . The detection range was 10-70 nM, and the limit of detection (S/N = 3) was 3.3 nM. In addition, this method could also realize the qualitative and semiquantitative detection of Hg according to the fluorescence colour change of the probe under ultraviolet light. After various evaluations, the method could be successfully applied to the quantitative and visual detection of Hg in tap water, and demonstrated excellent selectivity, anti-interference performance, and repeatability of the method.
本文基于汞离子（Hg ）对碳量子点（CQDs）荧光的猝灭作用以及对若丹明 B 荧光的无响应，构建了一种双发射比率荧光传感器，实现了 Hg 的定量检测。在 365nm 激发下，荧光光谱显示出两个发射峰，分别位于 437nm 和 590nm，分别对应于 CQDs 和若丹明 B 的荧光发射。该方法基于两个发射峰强度比 F/F 与 Hg 浓度之间的线性关系对 Hg 进行定量检测。检测范围为 10-70nM，检测限（S/N=3）为 3.3nM。此外，该方法还可以根据探针在紫外光下的荧光颜色变化实现对 Hg 的定性和半定量检测。经过各种评估，该方法可以成功应用于自来水中 Hg 的定量和可视化检测，并且表现出该方法出色的选择性、抗干扰性能和重现性。
