Long-term effect of loading on the fibroblast population of the periodontal ligament in the rat lower incisor.

The number and density of the cells in the tooth-related periodontal ligament (t-PDL) were related to the recovery of eruptive potential 2.5 months after load removal. Four left incisors in which eruption returned to normal (group A) and six in which eruption remained grossly impaired after a two-week application of latero-intrusive loads of 18.5 +/- 0.69 (group B) were compared to four control rats with unimpeded eruption. The incisors were demineralized, embedded in paraplast and cut into 6 micron transverse serial sections from which a three-dimensional reconstruction of the PDL was made by computerized histomorphometry, and the fibroblast population counted. The t-PDL volumes in controls and in groups A and B reached 1.15, 1.05 and 1.53 mm3, respectively; the estimated number of cells in the same PDL volumes were 2244 X 10(3), 1.659 X 10(3) and 2094 X 10(3) cells, respectively. The mean cell count/segment of PDL and the calculated number of cells/unit of periodontal tissue were significantly lower (p less than 0.001) in the formerly stressed, lateral periodontium of both experimental groups. In group B, these values were also decreased in the mesial periodontium.