成人费城染色体阳性急性淋巴细胞白血病中22号染色体断点的分子分析。

Molecular analysis of chromosome 22 breakpoints in adult Philadelphia-positive acute lymphoblastic leukaemia.

作者信息

Kurzrock R, Shtalrid M, Gutterman J U, Koller C A, Walters R, Trujillo J M, Talpaz M

机构信息

Department of Clinical Immunology, University of Texas System Cancer Center, M.D. Anderson Hospital and Tumor Institute at Houston 77030.

出版信息

Br J Haematol. 1987 Sep;67(1):55-9. doi: 10.1111/j.1365-2141.1987.tb02296.x.

DOI:10.1111/j.1365-2141.1987.tb02296.x

PMID:3478080

Abstract

The Philadelphia (Ph) translocation, t(9:22)(q 34:q11), is found in the majority of patients with chronic myelogenous leukaemia (CML) as well as in approximately 20% of adult acute lymphoblastic leukaemia (ALL) patients. The chromosome 22 breakpoint in CML has been localized within a restricted 5.8 kb segment of DNA known as the breakpoint cluster region (bcr). To investigate the chromosome 22 breakpoint in ALL, we analysed five adult Ph-positive ALL patients for bcr rearrangement. Rearrangement was detected within bcr in two patients. However, in one patient the break occurred 5' to the first exon of bcr and in two patients the bcr region was not involved. We conclude that the identical cytogenetic marker, t(9:22), may yield a different genomic configuration in ALL and CML.

摘要

费城（Ph）易位，即t(9;22)(q34;q11)，在大多数慢性粒细胞白血病（CML）患者以及约20%的成人急性淋巴细胞白血病（ALL）患者中被发现。CML中22号染色体的断点已定位在一段5.8 kb的限制性DNA片段内，该片段被称为断点簇区域（bcr）。为了研究ALL中22号染色体的断点，我们分析了5例成人Ph阳性ALL患者的bcr重排情况。在2例患者中检测到bcr内的重排。然而，1例患者的断点发生在bcr第一个外显子的5'端，2例患者的bcr区域未被累及。我们得出结论，相同的细胞遗传学标志物t(9;22)在ALL和CML中可能产生不同的基因组构型。