Wagner B, Schächtele C, Marmé D
Gödecke Research Institute, Freiburg, F.R.G.
Eur J Pharmacol. 1987 Aug 11;140(2):227-32. doi: 10.1016/0014-2999(87)90810-7.
The contractile effects of the phorbol-12,13-dibutyrate (PDBu) on rabbit basilar, renal, and saphenous arterial rings were investigated under various bathing conditions. At millimolar concentrations of Ca2+, the contractile responses with respect to both maximal contraction and sensitivity to PDBu were in the rank order basilar artery greater than renal artery greater than saphenous artery. Contractions were induced by PDBu also at micromolar concentrations of Ca2+. Depolarization of the cell membrane in the presence of micromolar Ca2+, which did not result in contraction of the vascular rings, caused a shift of the concentration response curves towards lower PDBu concentrations. These results are compatible with the assumption that PDBu induces contraction in vascular smooth muscle by activating protein kinase C.
在不同的浴槽条件下，研究了佛波醇-12,13-二丁酸酯（PDBu）对兔基底动脉、肾动脉和隐动脉环的收缩作用。在毫摩尔浓度的Ca2+条件下，就最大收缩和对PDBu的敏感性而言，收缩反应的顺序为基底动脉＞肾动脉＞隐动脉。在微摩尔浓度的Ca2+条件下，PDBu也能诱导收缩。在微摩尔Ca2+存在的情况下，细胞膜去极化并未导致血管环收缩，但却使浓度反应曲线向较低的PDBu浓度方向移动。这些结果与PDBu通过激活蛋白激酶C诱导血管平滑肌收缩的假设相符。
