通过流聚焦微流控装置制备载阿霉素的 PCL 纳米粒：包封效率和药物释放。

Production of doxorubicin-loaded PCL nanoparticles through a flow-focusing microfluidic device: encapsulation efficacy and drug release.

机构信息

Department of Chemical Engineering, University of Isfahan, Isfahan, Iran.

Department of Pharmaceutics, Isfahan University of Medical Sciences, Isfahan, Iran.

出版信息

Soft Matter. 2021 Dec 8;17(47):10675-10682. doi: 10.1039/d1sm01070k.

DOI:10.1039/d1sm01070k

PMID:34782908

Abstract

The present study shows a facile route for producing doxorubicin (DOX)-loaded polycaprolactone (PCL) nanoparticles using a microfluidic device with a flow-focusing platform in a single step. Indeed, the evaluation of the performance of the flow-focusing microfluidic device for the preparation of DOX-loaded PCL (DOX/PCL) nanoparticles with a uniform size distribution and high encapsulation efficiency (EE) by applying the liquid non-solvent precipitation process is very important. Accordingly, the physicochemical characteristics of the DOX/PCL nanoparticles such as their mean size, polydispersity index (PDI), and EE were investigated by studying different parameters such as the flow rate ratio (FRR) and DOX concentration. Also, the release study was carried out at two pH of 5.5 and 7.4. The mean size of DOX/PCL nanoparticles achieved was in the range of 120-320 nm with a PDI ≤ 0.29 and EE between 48% and 87%. Moreover, the release profile of DOX/PCL nanoparticles was sustained for 10 days (≤66%) at pH 7.4. This means that the production process can result in a high EE and low release of the DOX drug.

摘要

本研究展示了一种使用带有流聚焦平台的微流控装置一步法制备载多柔比星（DOX）聚己内酯（PCL）纳米粒子的简便方法。实际上，通过应用液相非溶剂沉淀法，评估用于制备具有均匀粒径分布和高包封效率（EE）的载 DOX-PCL（DOX/PCL）纳米粒子的流聚焦微流控装置的性能非常重要。因此，通过研究不同的参数，如流速比（FRR）和 DOX 浓度，研究了 DOX/PCL 纳米粒子的物理化学性质，如平均粒径、多分散指数（PDI）和 EE。此外，还在 pH 值为 5.5 和 7.4 的两种条件下进行了释放研究。所得 DOX/PCL 纳米粒子的平均粒径在 120-320nm 范围内，PDI≤0.29，EE 在 48%-87%之间。此外，DOX/PCL 纳米粒子的释放曲线在 pH 值为 7.4 时可持续 10 天（≤66%）。这意味着该生产工艺可实现高 EE 和低 DOX 药物释放。