Tecnologico de Monterrey, Department of Chemistry and Nanotechnology, School of Engineering and Science, Eugenio Garza Sada 2501 Sur, Col. Tecnologico, C.P. 64849 Monterrey, Nuevo León, Mexico.
Tecnologico de Monterrey, Department of Chemistry and Nanotechnology, School of Engineering and Science, Eugenio Garza Sada 2501 Sur, Col. Tecnologico, C.P. 64849 Monterrey, Nuevo León, Mexico.
Mater Sci Eng C Mater Biol Appl. 2020 Jul;112:110928. doi: 10.1016/j.msec.2020.110928. Epub 2020 Apr 3.
Nanofibers and smart polymers are potentially fascinating biomaterials for the sustained release of therapeutic agents and tissue engineering applications. The current study describes a new class of pH-controlled polycaprolactone/mercaptophenyl methacrylate functionalized carbon nano-onions (PCL/f-CNOs) composite nanofibers by Forcespinning® (FS) with a sustained drug release profile. The morphology and structural characteristics of PCL/f-CNOs nanofibers were scrutinized by Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR), respectively. The morphological results revealed that FS provided homogeneous and bead free nanofibers with average diameters from approximately 215 nm to 596 nm. PCL/f-CNOs composite fibers exhibited pH-responsive release of DOX over 15 days; pH 6.5 showed 87%, and pH 5.0 presented around 99% of DOX release. Drug release measurements showed that the π-π stacking interactions between DOX and f-CNOs have led to a controlled DOX release from forcespun PCL/f-CNOs fibers. Owing to the f-CNOs amalgamation, PCL/f-CNOs fibers unveiled enhanced tensile strength (3.16 MPa) as compared to pristine PCL fibers. It reveals the magnitude of colloidal stability and physisorption of f-CNOs within the PCL matrix. Besides, the in-vitro cell viability was measured with human fibroblast cells, and good viability was observed. Nevertheless, DOX embedded pH-responsive PCL/f-CNOs composite nanofibers may show potential applications in the biomedical research area.
纳米纤维和智能聚合物是具有潜力的生物材料，可用于治疗药物的持续释放和组织工程应用。本研究通过 Forcespinning®（FS）描述了一类新的 pH 控制的聚己内酯/巯基苯甲基丙烯酸酯功能化碳纳米洋葱（PCL/f-CNOs）复合纳米纤维，具有持续的药物释放特性。通过扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）分别对 PCL/f-CNOs 纳米纤维的形态和结构特征进行了研究。形态结果表明，FS 提供了均匀的、无珠的纳米纤维，平均直径约为 215nm 至 596nm。PCL/f-CNOs 复合纤维在 15 天内表现出 pH 响应性的 DOX 释放；pH6.5 时释放 87%，pH5.0 时释放约 99%的 DOX。药物释放测量表明，DOX 和 f-CNOs 之间的 π-π 堆积相互作用导致从 Forcespun PCL/f-CNOs 纤维中控制 DOX 释放。由于 f-CNOs 的结合，PCL/f-CNOs 纤维的拉伸强度（3.16MPa）比纯 PCL 纤维提高了。这揭示了 f-CNOs 在 PCL 基质中的胶体稳定性和物理吸附的程度。此外，通过人成纤维细胞测量了体外细胞活力，观察到良好的活力。然而，嵌入 DOX 的 pH 响应性 PCL/f-CNOs 复合纳米纤维可能在生物医学研究领域具有潜在的应用。
