Department of Restorative Dentistry, Dental Materials Division, Piracicaba Dental School, UNICAMP, State University of Campinas, Piracicaba, SP, Brazil.
Department of Restorative Dentistry and Biomaterials, University Medical Center Groningen, The University of Groningen, Center for Dentistry and Oral Hygiene, Groningen, The Netherlands.
Braz Dent J. 2021 Jul-Aug;32(4):106-115. doi: 10.1590/0103-6440202104463.
The aim of this study was to evaluate the effect of 2% chlorhexidine digluconate (CHX) on microtensile bond strength (µTBS) between an adhesive system and under 3 dentin conditions. For that, this study evaluated the adhesive interface at initial, after 6 months and 1 year of storage. Forty-eight human third molars were prepared and randomly divided into 3 groups, according to dentin substrates: sound dentin (Sd), caries-infected dentin (Ci) and caries-affected dentin (Ca). The groups were subdivided into two according to the dentin pre-treatment: application of 2% CHX or without pre-treatment (control). The dentin surfaces were etched with 35% phosphoric acid gel and bonded with Adper Single Bond 2 (3M ESPE) adhesive system according to manufacturer's instructions. Subsequently, the specimens were stored in deionized water at 37°C for 24h, 6 months and 1 year. Two additional teeth were used to analyze the bonding interfaces by SEM. Data was submitted to three-way ANOVA in a split plot design and Tukey's test (α = 0.05). The results showed that Ci decreased µTBS values when compared to Ca and Sd, regardless storages time or treatment. Stored samples for 6 months and 1 year decreased the µTBS for the control group, but no difference was found between storages time for the CHX group. As a conclusion, the 2% CHX application after etching showed improved dentin bond strength in the storage time, regardless of the substrates evaluated.
本研究旨在评估 2%葡萄糖酸氯己定（CHX）对 3 种牙本质条件下黏接系统微拉伸黏结强度（µTBS）的影响。为此，本研究评估了初始、6 个月和 1 年储存后的黏接界面。将 48 颗人第三磨牙制备并随机分为 3 组，根据牙本质底物：正常牙本质（Sd）、龋病感染牙本质（Ci）和龋病影响牙本质（Ca）。根据牙本质预处理将各组分为两组：应用 2%CHX 或不预处理（对照）。用 35%磷酸凝胶酸蚀牙本质表面，并按照制造商的说明用 Adper Single Bond 2（3M ESPE）黏接系统黏接。随后，将样本在去离子水中于 37°C 下储存 24h、6 个月和 1 年。另外两颗牙齿用于通过 SEM 分析黏接界面。数据采用三因素分组区组设计方差分析和 Tukey 检验（α=0.05）。结果表明，与 Ca 和 Sd 相比，Ci 降低了 µTBS 值，无论储存时间或处理方式如何。储存 6 个月和 1 年的样本降低了对照组的 µTBS，但 CHX 组的储存时间之间没有差异。结论是，酸蚀后应用 2%CHX 可在储存时间内提高牙本质黏结强度，而与评估的底物无关。
