Department of Chemistry and Chemical Biology, University of New Mexico, Albuquerque, New Mexico 87131, United States.
Center for Biomedical Engineering, University of New Mexico, Albuquerque, New Mexico 87131, United States.
ACS Appl Mater Interfaces. 2021 Dec 1;13(47):55953-55965. doi: 10.1021/acsami.1c18553. Epub 2021 Nov 17.
The threat of antibiotic-resistant bacteria is an ever-increasing problem in public health. In this report, we examine the photochemical properties with a proof-of-principle biocidal assay for a novel series of regio-regular imidazolium derivative poly-(3-hexylthiophene)/sodium dodecyl sulfate (P3HT-Im/SDS) materials from ultrafast sub-ps dynamics to μs generation of reactive oxygen species (ROS) and 30 min biocidal reactivity with (). This broad series encompassing pure P3HT-Im to cationic, neutral, and anionic P3HT-Im/SDS materials are all interrogated by a variety of techniques to characterize the physical material structure, electronic structure, and antimicrobial activity. Our results show that SDS complexation with P3HT-Im results in aggregate materials with reduced ROS generation and light-induced anti-microbial activity. However, our characterization reveals that the presence of non-aggregated or lightly SDS-covered polymer segments is still capable of ROS generation. Full encapsulation of the P3HT-Im polymer completely deactivates the light killing pathway. High SDS concentrations, near and above critical micelle concentration, further deactivate all anti-microbial activity (light and dark) even though the P3HT-Im regains its electronic properties to generate ROS.
抗生素耐药菌的威胁是公共卫生领域日益严重的问题。在本报告中，我们从超快亚皮秒动力学到μs 时间尺度上的活性氧（ROS）生成，以及 30 分钟的（）生物杀灭反应，对一系列新型区域规整的咪唑鎓衍生物聚（3-己基噻吩）/十二烷基硫酸钠（P3HT-Im/SDS）材料的光化学性质进行了检验，建立了一个原理验证的杀菌测定法。这一系列广泛的材料包括纯 P3HT-Im 到阳离子、中性和阴离子 P3HT-Im/SDS 材料，都通过多种技术进行了测试，以表征其物理材料结构、电子结构和抗菌活性。我们的结果表明，SDS 与 P3HT-Im 复合会导致聚合材料的 ROS 生成和光诱导抗菌活性降低。然而，我们的特性分析表明，非聚集或轻度 SDS 覆盖的聚合物段仍然能够产生 ROS。P3HT-Im 聚合物的完全包裹会使光杀伤途径失活。即使 P3HT-Im 恢复其产生 ROS 的电子性质，高 SDS 浓度（接近或高于临界胶束浓度）也会使所有抗菌活性（光照和黑暗）失活。
