promotes breast cancer via regulating .

BACKGROUND

Breast cancer (BC) is the most common cancer diagnosed among women and is the second leading cause of cancer death. It is of great significance to explore potential candidate targets for BC.

METHODS

The expression of ubiquitin-specific protease 41 () and its prognosis prediction function was firstly evaluated by TCGA database analysis. Using BC cell lines and specimens from 10 patients with primary BC, the upregulation of in BC was ensured. By overexpression or knockdown, its function was studied by cell function assays, small interfering RNA (), western blot, mass spectrometry, and flow cytometry. The potential mechanism of was explored via Co-Immunoprecipitation mass spectrometry, and western blot.

RESULTS

TCGA database analysis revealed that in metastatic BC, expression was upregulated and negatively correlated with BC prognosis. In BC cancer cells and cancer specimens, was also upregulated. Overexpression of greatly enhanced BC colony-forming ability, proliferation, and migration. In contrast, knockdown significantly inhibited BC colony-forming ability, proliferation, and migration. Moreover, Co-Immunoprecipitation mass spectrometry results indicated that could interact with . promoted the protein expression of . The expression of in BC tissues was upregulated. Knockdown of inhibited cell growth and migration, and reversed the oncogenic function of in BC cells.

CONCLUSIONS

can be a potential therapeutic target against BC via .