Ameloot P C, Coomans D
Tandheelkundig Instituut, Vrije Universiteit Brussel, Belgium.
Arch Oral Biol. 1987;32(6):381-9. doi: 10.1016/0003-9969(87)90072-0.
The effect of addition of eight different combinations of Ca, Mg and P supplements (control, Ca, Mg, P, CaMg, CaP, PMg and CaMgP) on three-day-old rat maxillary second molars, explanted at the premineralizing stage and cultured for two weeks, was studied. Light-microscopy sections, cut parallel to the occlusal plane, were divided into four sectors and given a score according to an ordinal scale for dentine and enamel depending on the regularity of these matrices. An analysis of variance on these scores revealed a significant favourable effect of Mg, CaMg and CaMgP and an adverse effect of Ca on enamel. A favourable effect on dentine regularity was obtained after addition of Ca or Mg. Ultrastructurally, enamel changes such as amorphous enamel matrix, voids and disturbance in rod-interrod pattern were seen after addition of Ca, P, CaP. Thin enamel with less tight packing of crystals was observed after CaMg addition. A thick layer of enamel with highly-organized rod-interrod pattern was seen with Mg, PMg and CaMgP addition. It is suggested that Mg plays an important role in the interaction with Ca and P for the harmonious development of enamel and dentine in vitro.
研究了添加钙（Ca）、镁（Mg）和磷（P）的八种不同组合（对照、Ca、Mg、P、CaMg、CaP、PMg和CaMgP）对矿化前期离体培养两周的三日龄大鼠上颌第二磨牙的影响。平行于咬合面切割的光学显微镜切片被分为四个区域，并根据牙本质和牙釉质基质的规则性，按照顺序量表给出分数。对这些分数进行方差分析发现，Mg、CaMg和CaMgP具有显著的有利影响，而Ca对牙釉质有不利影响。添加Ca或Mg后，牙本质规则性得到有利影响。在超微结构上，添加Ca、P、CaP后可见牙釉质变化，如无定形牙釉质基质、空隙和釉柱-柱间质模式紊乱。添加CaMg后观察到牙釉质变薄，晶体排列不紧密。添加Mg、PMg和CaMgP后可见一层厚的牙釉质，具有高度组织化的釉柱-柱间质模式。研究表明，Mg在与Ca和P的相互作用中发挥重要作用，以促进体外牙釉质和牙本质的和谐发育。
