Hu Fang-Xiao, Yang Jie, Yang Chuan-Hua, Tao Yan-Nan, Yang Xue-Song, Cui Ming-Ling, Li Guan-Lan, Li Chao, Jiang Yue-Hua
Shandong University of Traditional Chinese Medicine, Jinan, China.
Department of Cardiovascular, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China.
Ann Transl Med. 2021 Oct;9(20):1589. doi: 10.21037/atm-21-5176.
Increasingly, evidence has shown that long non-coding RNAs (lncRNAs) play an important role in isolated systolic hypertension (ISH). However, a systematic lncRNA-messenger RNA (mRNA) regulatory network is still absent in isolated systolic hypertension and atherosclerotic cerebral infarction patients (ISH & ACI). This research aimed to establish a lncRNA-mRNA co-expression network in patients with ISH & ACI, to probe into the potential functions of lncRNA in such patients.
Expression profiles of lncRNA and mRNAs were collected and compared, from 8 patients with ISH and 8 patients with ISH & ACI by RNA-seq data. Differentially expressed lncRNAs and mRNAs were screened out via high-throughput sequencing in the plasma of ISH/ACI patients and control ISH patients. Then, a lncRNA-mRNA interaction network was built using the Pearson correlation coefficient by Cytoscape software. The expression levels of the hub genes and lncRNAs were verified by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) in another 10 ISH/ACI patients and 10 control patients. This study was approved by the responsible institutional review board (IRB) and informed consent was provided by participants.
A total of 2,768 differentially expressed lncRNAs and 747 differentially expressed mRNAs were identified. We identified two hub genes ( and ) and 11 lncRNAs in the lncRNA-mRNA interaction network. The results of qRT-PCR and cell assay verified that lncRNAs ENST00000590604 and CD226 are highly expressed in patients of ISH & ACI. Further, CD226 was associated with vascular endothelial cells growth and stability through the platelet activation and focal adhesion pathway.
We established a novel mRNA-lncRNA interaction network. The lncRNAs ENST00000590604 and CD226 might be the potential biomarkers of ISH & ACI.
越来越多的证据表明，长链非编码RNA（lncRNA）在单纯收缩期高血压（ISH）中发挥重要作用。然而，在单纯收缩期高血压和动脉粥样硬化性脑梗死患者（ISH & ACI）中，仍缺乏系统的lncRNA-信使核糖核酸（mRNA）调控网络。本研究旨在建立ISH & ACI患者的lncRNA-mRNA共表达网络，以探究lncRNA在此类患者中的潜在功能。
通过RNA测序数据收集并比较8例ISH患者和8例ISH & ACI患者的lncRNA和mRNA表达谱。通过高通量测序筛选ISH/ACI患者和对照ISH患者血浆中差异表达的lncRNA和mRNA。然后，使用Pearson相关系数通过Cytoscape软件构建lncRNA-mRNA相互作用网络。在另外10例ISH/ACI患者和10例对照患者中，通过定量逆转录-聚合酶链反应（qRT-PCR）验证枢纽基因和lncRNA的表达水平。本研究经负责的机构审查委员会（IRB）批准，参与者均提供了知情同意书。
共鉴定出2768个差异表达的lncRNA和747个差异表达的mRNA。我们在lncRNA-mRNA相互作用网络中鉴定出两个枢纽基因（和）和11个lncRNA。qRT-PCR和细胞实验结果证实，lncRNA ENST00000590604和CD226在ISH & ACI患者中高表达。此外，CD226通过血小板活化和粘着斑途径与血管内皮细胞生长和稳定性相关。
我们建立了一个新的mRNA-lncRNA相互作用网络。lncRNA ENST00000590604和CD226可能是ISH & ACI的潜在生物标志物。
