Jathar Sonali, Kumar Vikram, Srivastava Juhi, Tripathi Vidisha
National Centre for Cell Science, Pune University Campus, Ganeshkhind, Pune, 411007, India.
Adv Exp Med Biol. 2017;1008:283-323. doi: 10.1007/978-981-10-5203-3_10.
It is estimated that more than 90% of the mammalian genome is transcribed as non-coding RNAs. Recent evidences have established that these non-coding transcripts are not junk or just transcriptional noise, but they do serve important biological purpose. One of the rapidly expanding fields of this class of transcripts is the regulatory lncRNAs, which had been a major challenge in terms of their molecular functions and mechanisms of action. The emergence of high-throughput technologies and the development in various conventional approaches have led to the expansion of the lncRNA world. The combination of multidisciplinary approaches has proven to be essential to unravel the complexity of their regulatory networks and helped establish the importance of their existence. Here, we review the current methodologies available for discovering and investigating functions of long non-coding RNAs (lncRNAs) and focus on the powerful technological advancement available to specifically address their functional importance.
据估计，超过90%的哺乳动物基因组被转录为非编码RNA。最近的证据表明，这些非编码转录本并非垃圾或仅仅是转录噪音，而是具有重要的生物学功能。这类转录本中迅速发展的一个领域是调控性长链非编码RNA（lncRNA），其分子功能和作用机制一直是个重大挑战。高通量技术的出现以及各种传统方法的发展，推动了lncRNA领域的扩展。多学科方法的结合已被证明对于揭示其调控网络的复杂性至关重要，并有助于确立其存在的重要性。在此，我们综述了目前可用于发现和研究长链非编码RNA（lncRNA）功能的方法，并重点介绍了可专门阐明其功能重要性的强大技术进展。
