Chatterjee A, Freeman J W, Busch H
Baylor College of Medicine, Department of Pharmacology, Houston, Texas 77030.
Cancer Res. 1987 Dec 1;47(23):6329-34.
This study reports the identification and partial characterization of a novel Mr 105,000 nucleolar antigen (P105) identified by a monoclonal antibody. This monoclonal antibody was obtained when a nucleolar protein extract separated from the immunodominant protein C23 was used as the immunogen. Nucleolar antigen P105 was not detected in normal (resting) human liver, kidney, or peripheral blood lymphocytes but was present in a variety of human malignant cells and tissues. Lymphocyte nucleoli also exhibited specific P105 staining after 72 h of phytohemagglutinin stimulation. Nucleolar antigen P105 was detected in growing and dividing HL 60 cells but was not detected in retinoic acid-induced differentiated HL 60 cells. When HeLa cells were made quiescent by 48 h of serum starvation, the P105 antigen was not detected, but after refeeding with serum-containing medium, the antigen P105 was detected in the HeLa nucleoli within 2 h. These results indicate that nucleolar antigen P105 is a proliferating cell nuclear and nucleolar antigen-like molecule which appears early in the G1-S phase of the cell cycle.
本研究报告了一种由单克隆抗体鉴定的新型105,000 Mr核仁抗原（P105）及其部分特性。当从免疫显性蛋白C23分离得到的核仁蛋白提取物用作免疫原时，获得了这种单克隆抗体。在正常（静止）人肝脏、肾脏或外周血淋巴细胞中未检测到核仁抗原P105，但在多种人类恶性细胞和组织中存在。在植物血凝素刺激72小时后，淋巴细胞核仁也表现出特异性P105染色。在生长和分裂的HL 60细胞中检测到核仁抗原P105，但在维甲酸诱导分化的HL 60细胞中未检测到。当HeLa细胞通过48小时血清饥饿进入静止期时，未检测到P105抗原，但在用含血清培养基重新培养后，在2小时内HeLa核仁中检测到抗原P105。这些结果表明，核仁抗原P105是一种增殖细胞核和核仁抗原样分子，在细胞周期的G1-S期早期出现。
