Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi.
Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool, UK.
Microb Genom. 2021 Nov;7(11). doi: 10.1099/mgen.0.000703.
A special-care neonatal unit from a large public hospital in Malawi was noted as having more frequent, difficult-to-treat infections, and a suspected outbreak of multi-drug-resistant was investigated using genomic characterisation. All bloodstream infections (BSIs) from patients in the neonatal ward (=62), and a subset of BSI isolates (=38) from other paediatric wards in the hospital, collected over a 4 year period were studied. After whole genome sequencing, the strain sequence types (STs), plasmid types, virulence and resistance genes were identified. One ST340 clone, part of clonal complex 258 (CC258) and an ST that drives hospital outbreaks worldwide, harbouring numerous resistance genes and plasmids, was implicated as the likely cause of the outbreak. This study contributes molecular information necessary for tracking and characterizing this important hospital pathogen in sub-Saharan Africa.
马拉维一家大型公立医院的特别护理新生儿病房被发现感染更为频繁且难以治疗，疑似爆发了多药耐药。使用基因组特征对其进行了调查。对来自新生儿病房的所有 62 名患者的血流感染（BSI）以及医院其他儿科病房的 BSI 分离株的亚组（38 株）进行了为期 4 年的研究。进行全基因组测序后，鉴定了菌株序列类型（ST）、质粒类型、毒力和耐药基因。一个 ST340 克隆，属于克隆复合体 258（CC258）的一部分，也是导致全球医院爆发的 ST，携带众多耐药基因和质粒，被认为是此次爆发的可能原因。本研究为追踪和描述撒哈拉以南非洲地区这种重要医院病原体提供了必要的分子信息。
