School of Pharmacy, Jiangsu University, Zhenjiang 212013, People's Republic of China.
The People's Hospital of Danyang, Affiliated Danyang Hospital of Nantong University, Zhenjiang 212300, People's Republic of China.
Nanotechnology. 2021 Dec 6;33(9). doi: 10.1088/1361-6528/ac3b0e.
A magnetically induced self-assembly DNAzyme electrochemical biosensor based on gold-modified-FeO/FeOheterogeneous nanoparticles was successfully fabricated to detect Nickel(II) (Ni). The phase composition and magnetic properties of-FeO/FeOheterogeneous nanoparticles controllably prepared by the citric acid (CA) sol-gel method were investigated in detail. The-FeO/FeOheterogeneous nanoparticles were modified by using trisodium citrate as reducing agent, and the magnetically induced self-assembly-FeO/FeO-Au nanocomposites were obtained. The designed Ni-dependent DNAzyme consisted of the catalytic chain modified with the thiol group (S1-SH) and the substrate chain modified with methylene blue (S2-MB). The MGCE/-FeO/FeO-Au/S1/BSA/S2 electrochemical sensing platform was constructed and differential pulse voltammetry was applied for electrochemical detection. Under the optimum experimental parameters, the detection range of the biosensor was 100 pM-10M ( = 0.9978) with the limit of detection of 55 pM. The biosensor had high selectivity, acceptable stability, and reproducibility (RSD = 4.03%).
基于金修饰的 FeO/FeO 纳米异质粒子的磁诱导自组装 DNA zyme 电化学生物传感器成功制备，用于检测镍（II）（Ni）。通过柠檬酸（CA）溶胶-凝胶法详细研究了可控制备的-FeO/FeO 纳米异质粒子的物相组成和磁性能。-FeO/FeO 纳米异质粒子通过使用三钠柠檬酸作为还原剂进行修饰，得到磁诱导自组装-FeO/FeO-Au 纳米复合材料。设计的 Ni 依赖性 DNA 酶由巯基（S1-SH）修饰的催化链和亚甲基蓝（S2-MB）修饰的底物链组成。构建了 MGCE/-FeO/FeO-Au/S1/BSA/S2 电化学传感平台，并应用差分脉冲伏安法进行电化学检测。在最佳实验参数下，该生物传感器的检测范围为 100 pM-10 M（r=0.9978），检测限为 55 pM。该生物传感器具有高选择性、良好的稳定性和可重复性（RSD=4.03%）。
