Hacıoglu Seda, Kunduhoglu Buket
Institute of Science, University of Eskişehir Osmangazi, Eskişehir 26040, Turkey.
Department of Biology, Faculty of Science and Letters, University of Eskişehir Osmangazi, Eskişehir 26040, Turkey.
Food Sci Anim Resour. 2021 Nov;41(6):967-982. doi: 10.5851/kosfa.2021.e49. Epub 2021 Nov 1.
Probiotics are living microorganisms that, when administered in adequate amounts, provide a health benefit to the host and are considered safe. Most probiotic strains that are beneficial to human health are included in the "Lactic acid bacteria" (LAB) group. The positive effects of probiotic bacteria on the host's health are species-specific and even strain-specific. Therefore, evaluating the probiotic potential of both wild and novel strains is essential. In this study, the probiotic characteristics of KT38-3 were determined. The strain identification was achieved by 16S rRNA sequencing. API-ZYM test kits were used to determine the enzymatic capacity of the strain. KT38-3 was able to survive in conditions with a broad pH range (pH 2-7), range of bile salts (0.3%-1%) and conditions that simulated gastric juice and intestinal juice. The percentage of autoaggregation (59.4%), coaggregation with O157:H7 (37.4%) and hydrophobicity were determined to be 51.1%, 47.4%, and 52.7%, respectively. KT38-3 produced β-galactosidase enzymes and was able ferment lactose. In addition, this strain was capable of producing antimicrobial peptides against the bacteria tested, including methicillin and/or vancomycin-resistant bacteria. The cell-free supernatants of the strain had high antioxidant activities (DPPH: 54.9% and ABTS: 48.7%). Therefore, considering these many essential probiotic properties, KT38-3 has the potential to be used as a probiotic supplement. Supporting these findings with experiments to evaluate the potential health benefits will be the subject of our future work.
益生菌是一类活的微生物，当给予足够数量时，能给宿主带来健康益处且被认为是安全的。大多数对人类健康有益的益生菌菌株都属于“乳酸菌”（LAB）组。益生菌对宿主健康的积极作用具有种属特异性甚至菌株特异性。因此，评估野生菌株和新菌株的益生菌潜力至关重要。在本研究中，测定了KT38 - 3的益生菌特性。通过16S rRNA测序实现菌株鉴定。使用API - ZYM测试试剂盒测定该菌株的酶活性。KT38 - 3能够在较宽的pH范围（pH 2 - 7）、胆汁盐范围（0.3% - 1%）以及模拟胃液和肠液的条件下存活。其自聚集率（59.4%）、与O157:H7的共聚集率（37.4%）和疏水性分别测定为51.1%、47.4%和52.7%。KT38 - 3产生β - 半乳糖苷酶并能够发酵乳糖。此外，该菌株能够产生针对所测试细菌的抗菌肽，包括耐甲氧西林和/或耐万古霉素的细菌。该菌株的无细胞上清液具有较高的抗氧化活性（DPPH：54.9%和ABTS：48.7%）。因此，考虑到这些众多重要的益生菌特性，KT38 - 3有潜力用作益生菌补充剂。通过实验评估其潜在的健康益处来支持这些发现将是我们未来工作的主题。
