Department of Urology, Nara Medical University, Nara, Japan.
Tsumura Kampo Research Laboratories, Kampo Research and Development Division, Tsumura & Co, Tokyo, Japan.
Low Urin Tract Symptoms. 2022 Mar;14(2):122-128. doi: 10.1111/luts.12418. Epub 2021 Nov 18.
This study aimed to determine whether Dahl salt-sensitive rats fed a high-salt diet would show features of nocturia due to nocturnal polyuria and to examine the efficacy of choreito (CRT) on nocturnal polyuria.
Dahl salt-sensitive rats were divided into three groups. Group A was fed a 4% salt diet, group B a 2% salt diet, and group C a normal 0.3% salt diet. In groups α and β, other rats were further divided into two groups: The rats in group α were fed a 2% salt plus 3% CRT diet, and those in group β, were fed a 2% salt diet. Each rat was placed in an individual metabolic cage for 24 hours every week for 6 weeks. Water intake, urine production, voiding frequency, and voided volume per micturition were recorded.
The systolic blood pressure increased in the group fed a 4% salt diet compared to groups fed with a 2% and 0.3% salt diet. The urinary volume was higher in the groups fed with 4% and 2% salt than in the group fed with 0.3% salt. Further, water intake in the group fed a 2% salt plus 3% CRT diet was significantly lower than that in the group fed with a 2% salt diet.
Dahl salt-sensitive rats fed a 2% salt diet were candidates for a model of nocturnal polyuria. Using this model, we suggest that CRT reduces water intake in the active phase and contributes to water restriction in the treatment of nocturnal polyuria.
本研究旨在确定给予高盐饮食的 Dahl 盐敏感大鼠是否会因夜间多尿而出现夜尿症的特征，并检验 choreito（CRT）对夜间多尿的疗效。
将 Dahl 盐敏感大鼠分为三组。A 组给予 4%盐饮食，B 组给予 2%盐饮食，C 组给予正常 0.3%盐饮食。在组 α 和组 β 中，其他大鼠进一步分为两组：组 α 中的大鼠给予 2%盐加 3%CRT 饮食，组 β 中的大鼠给予 2%盐饮食。每周每组大鼠各置于单独代谢笼中 24 小时，共 6 周。记录水摄入量、尿量、排尿频率和每次排尿量。
与给予 2%和 0.3%盐饮食的大鼠相比，给予 4%盐饮食的大鼠收缩压升高。给予 4%和 2%盐饮食的大鼠尿量高于给予 0.3%盐饮食的大鼠。此外，给予 2%盐加 3%CRT 饮食的大鼠的水摄入量明显低于给予 2%盐饮食的大鼠。
给予 2%盐饮食的 Dahl 盐敏感大鼠是夜间多尿模型的候选者。使用该模型，我们建议 CRT 减少活动期的水摄入量，并有助于限制夜间多尿的治疗中的水摄入。
