Department of Nail and Breast Surgery, Tongxiang First People's Hospital, Zhejiang 314500, China.
Department of General Surgery, Tongxiang First People's Hospital, Zhejiang 314500, China.
Biochem Cell Biol. 2022 Feb;100(1):85-92. doi: 10.1139/bcb-2021-0275. Epub 2021 Nov 19.
This study characterized the effects of artesunate on thyroid cancer and partially identified the related molecular mechanisms. We determined the effect of artesunate on the proliferation of thyroid cancer cells using the MTT assay, cell colony formation experiments, and western blotting, and used flow cytometry to detect the apoptosis of cancer cells. Using a wound healing assay, Transwell chamber experiments, and western blotting, we determined the effect of artesunate on cancer cell migration. We also partially identified the molecular mechanism by co-cultivation of artesunate with the PI3K agonist 740Y-P. Artesunate significantly inhibited the growth, proliferation, migration, and invasion of thyroid cancer cells and promoted the apoptosis of cancer cells. Using co-cultivation with a PI3K agonist, we found that the inhibitory effect of artesunate on cancer cells was mainly due to suppression of the PI3K/AKT/FKHR signaling pathway. By inhibiting the PI3K/AKT/FKHR signaling pathway, artesunate induced apoptosis in thyroid cancer cells and inhibited their proliferation and migration.
本研究探讨了青蒿琥酯对甲状腺癌的作用，并部分阐明了相关的分子机制。我们通过 MTT 检测、细胞集落形成实验和 Western blot 检测，来确定青蒿琥酯对甲状腺癌细胞增殖的影响，并用流式细胞术检测癌细胞的凋亡。通过划痕愈合实验、Transwell 室实验和 Western blot 检测，来确定青蒿琥酯对癌细胞迁移的影响。我们还通过与 PI3K 激动剂 740Y-P 共培养，部分阐明了分子机制。青蒿琥酯显著抑制甲状腺癌细胞的生长、增殖、迁移和侵袭，促进癌细胞凋亡。通过与 PI3K 激动剂共培养，我们发现青蒿琥酯对癌细胞的抑制作用主要是由于抑制了 PI3K/AKT/FKHR 信号通路。通过抑制 PI3K/AKT/FKHR 信号通路，青蒿琥酯诱导甲状腺癌细胞凋亡，并抑制其增殖和迁移。
