College of Tropical Crops, Hainan University, Haikou, Hainan, P.R. China.
Coconut Research Institute, Chinese Academy of Tropical Agricultural sciences, Wenchang, Hainan, P.R. China.
PLoS One. 2021 Nov 19;16(11):e0259684. doi: 10.1371/journal.pone.0259684. eCollection 2021.
Vitamin E is essential for human health and plays positive roles in anti-oxidation. Previously, we detected large variation in vitamin E content among 161 oil palm accessions. In this study, twenty oil palm accessions with distinct variation in vitamin E contents (171.30 to 1 258.50 ppm) were selected for genetic variation analysis and developing functional markers associated with vitamin E contents. Thirty-seven homologous genes in oil palm belonging to vitamin E biosynthesis pathway were identified via BLASTP analysis, the lengths of which ranged from 426 to 25 717 bp (average 7 089 bp). Multiplex PCR sequencing for the 37 genes found 1 703 SNPs and 85 indels among the 20 oil palm accessions, with 226 SNPs locating in the coding regions. Clustering analysis for these polymorphic loci showed that the 20 oil palm accessions could be divided into five groups. Among these groups, group I included eight oil palm accessions whose vitamin E content (mean value: 893.50 ppm) was far higher than other groups (mean value 256.29 to 532.94 ppm). Correlation analysis between the markers and vitamin E traits showed that 134 SNP and 7 indel markers were significantly (p < 0.05) related with total vitamin E content. Among these functional markers, the indel EgTMT-1-24 was highly correlated with variation in vitamin E content, especially tocotrienol content. Our study identified a number of candidate function associated markers and provided clues for further research into molecular breeding for high vitamin E content oil palm.
维生素 E 对人类健康至关重要，在抗氧化方面发挥着积极作用。此前，我们在 161 份油棕品种中检测到维生素 E 含量存在较大差异。在这项研究中，我们选择了 20 份维生素 E 含量差异较大（171.30 至 1258.50ppm）的油棕品种进行遗传变异分析，并开发与维生素 E 含量相关的功能标记。通过 BLASTP 分析，在油棕中鉴定了 37 个属于维生素 E 生物合成途径的同源基因，其长度范围从 426 到 25717bp（平均 7089bp）。对这 37 个基因进行多重 PCR 测序，在 20 个油棕品种中发现了 1703 个 SNPs 和 85 个插入缺失，其中 226 个 SNPs 位于编码区。对这些多态性位点的聚类分析表明，这 20 个油棕品种可以分为 5 组。在这些组中，第 1 组包括 8 个油棕品种，其维生素 E 含量（平均值：893.50ppm）远高于其他组（平均值 256.29 至 532.94ppm）。标记与维生素 E 性状的相关分析表明，134 个 SNP 和 7 个插入缺失标记与总维生素 E 含量显著相关（p<0.05）。在这些功能标记中，EgTMT-1-24 插入缺失与维生素 E 含量的变化高度相关，特别是生育三烯酚的含量。本研究鉴定了一些与高维生素 E 含量油棕相关的候选功能标记，为进一步开展高维生素 E 含量油棕的分子育种研究提供了线索。
