Effects of storage temperature and time on enzymatic digestibility and fermentability of Densifying lignocellulosic biomass with chemicals pretreated corn stover.

A novel pretreatment, Densifying Lignocellulosic biomass with acidic/alkali Chemicals (DLC), was recently invented and owns unique advantages for biomass logistics and fermentation. The pretreatment was largely completed during biomass storage, which renders the storage conditions critical. In this study, the effects of storage temperature (-80 °C to 60 °C) and storage time (up to half a year) on the enzymatic digestibility and fermentability of DLC corn stover (CS) were investigated. DLC-CS containing calcium hydroxide(ch) showed increased enzymatic digestibility with increased storage temperature and time. High glucan conversions (>90%) and ethanol titers (e.g. 73.1 g/L) were achieved after regular steam autoclave of DLC(ch)-CS, without washing or detoxification. DLC-CS containing sulfuric acid(sa) was sensitive to storage conditions, and autoclaved DLC(sa)-CS reached the highest ethanol titer (66.6 g/L) when DLC(sa)-CS was stored at room temperature for 14 days. Results indicated that different ambient temperatures in different regions and seasons have a far-reaching impact on DLC-CS for bioconversion.