Department of Biotechnology, Assam University, Silchar 788011, Assam, India.
Department of Biotechnology, Assam University, Silchar 788011, Assam, India.
Infect Genet Evol. 2021 Dec;96:105139. doi: 10.1016/j.meegid.2021.105139. Epub 2021 Nov 16.
A small non-coding, evolutionarily conserved regulatory RNA molecule known as microRNA (miRNA) regulates various cellular activities and pathways. MicroRNAs remain evolutionarily conserved in different species of same taxa. They are present in all organisms including viruses. Viral miRNAs are small, less conserved and less stable and have higher negative minimal folding free energy than miRNAs of different organisms. The size of viral precursor miRNA is approximately 60-119 nucleotides in length. The structure of the mature miRNA sequences is predicted by using higher negative MFE (ΔG) value. Rous sarcoma Virus (RSV), named after its inventor Peyton Rous, has been known for causing tumors in the chicken for which it is known as an oncogenic retrovirus. Using specific criteria we have predicted 5 potential miRNAs in RSV which targeted 8 tumor suppressor genes in Gallus gallus. This study aims to predict the potential miRNAs, secondary structures and their targets for better understanding of the regulatory network of Rous sarcoma virus miRNA in forming sarcoma.
一种被称为 microRNA（miRNA）的小非编码、进化上保守的调节性 RNA 分子，调节着各种细胞活动和途径。miRNA 在同一分类群的不同物种中保持进化上的保守性。它们存在于包括病毒在内的所有生物体中。病毒 miRNA 较小，保守性较低，稳定性较低，负最小折叠自由能（ΔG）值高于不同生物体的 miRNA。病毒前体 miRNA 的大小约为 60-119 个核苷酸。成熟 miRNA 序列的结构通过使用更高的负 MFE（ΔG）值来预测。劳斯肉瘤病毒（RSV），以其发明者 Peyton Rous 的名字命名，因其在鸡中引起肿瘤而被称为致癌逆转录病毒。使用特定标准，我们预测了 RSV 中的 5 个潜在 miRNA，这些 miRNA 靶向 Gallus gallus 中的 8 个肿瘤抑制基因。本研究旨在预测潜在的 miRNA、二级结构及其靶标，以更好地理解 Rous sarcoma virus miRNA 在形成肉瘤中的调控网络。
