Menanteau J, Mitre D, Raher S
U.225 INSERM, Faculté de Chirurgie Dentaire, Nantes, France.
Arch Oral Biol. 1986;31(12):807-10. doi: 10.1016/0003-9969(86)90132-9.
An immature enamel fraction, as far as possible without cells, was prepared from fetal bovine molars, using aqueous-density fractionation. Portions were incubated at 37 degrees C with or without protease inhibitors. Amelogenins and enamelins were then examined for their molecular weight using HPLC-gel permeation. Degradation of amelogenins occurred rapidly and appeared to be related to proteolytic activity, probably localized extra-cellularly. Enamelins remained almost stable over the time intervals used.
采用水相密度分级法从胎牛磨牙制备尽可能无细胞的未成熟牙釉质部分。将部分样品在37℃下分别在有或无蛋白酶抑制剂的条件下孵育。然后使用高效液相色谱 - 凝胶渗透法检测釉原蛋白和釉蛋白的分子量。釉原蛋白迅速降解，似乎与蛋白水解活性有关，可能定位于细胞外。在所使用的时间间隔内，釉蛋白几乎保持稳定。
