Heinsohn R, Seitz C R, Hollinshead A C, Hyun W, Poschmann A
Universitätskrankenhaus Eppendorf Kinderklinik, Abteilung für Klinische Immunpathologie, Hamburg, Federal Republic of Germany.
Cancer Detect Prev Suppl. 1987;1:235-9.
Membranes were prepared from mammary tumors and fractionized using gel filtration and gradient polyacrylamide gel electrophoresis. To obtain monoclonal antibodies against tumor-associated antigens, mice were immunized against membrane fraction 2a. After hybridization, we obtained 746 hybridoma cell lines. We performed the immunodot assay for screening. Every supernatant was tested against 15 antigens. Eight antigens were purified fractions from tumor membrane preparation, and five were crude membrane preparations from benign and malignant breast cell lines. Additionally, the Thomsen-Friedenreich antigen of erythrocytes was tested. We selected 83 hybridomas for further characterization.
从乳腺肿瘤制备细胞膜，并使用凝胶过滤和梯度聚丙烯酰胺凝胶电泳进行分级分离。为了获得针对肿瘤相关抗原的单克隆抗体，用膜组分2a免疫小鼠。杂交后，我们获得了746个杂交瘤细胞系。我们进行免疫斑点试验进行筛选。每个上清液针对15种抗原进行检测。8种抗原是肿瘤膜制备物的纯化组分，5种是良性和恶性乳腺细胞系的粗制膜制备物。此外，还检测了红细胞的汤姆森-弗里德赖希抗原。我们选择了83个杂交瘤进行进一步鉴定。
