Lang E, Altevogt P, Schirrmacher V
Institut für Immunologie und Genetik, Deutsches Krebsforschungszentrum, Heidelberg, Federal Republic of Germany.
Cancer Detect Prev Suppl. 1987;1:73-9.
A highly metastatic murine tumor line (ESb) and a plastic-adherent variant derived from it (ESb-M) were compared for expression of cell surface glycoproteins. Previous studies had shown that ESb-M cells were very similar to ESb cells in terms of cell surface marker expression and invasive capacity in vitro, but studies in vivo revealed that they exerted a decreased metastatic capacity. Syngeneic animals inoculated SC with ESb-M cells developed larger primary tumors and survived much longer than animals inoculated similarly with ESb cells. When using the lectin soybean agglutinin (SBA), distinct differences were observed in the glycosylation of a 220 kDa and a 80 kDa cell surface glycoprotein. Further differences in expression of cell membrane proteins were detected by means of variant-specific monoclonal antibodies. These specific ligands reacted with 65-75 kDa membrane glycoproteins, which were more prominent in ESb-M cells than in ESb cells. Apart from these differences, the two cell lines showed very similar profiles of membrane glycoproteins and lectin staining. Whether the structural differences seen in cell surface proteins can explain the changes in functional behavior (metastatic behavior and plastic-adhesive properties) of the cells remains to be investigated.
对一种高转移性小鼠肿瘤细胞系（ESb）及其衍生的贴壁变异株（ESb-M）的细胞表面糖蛋白表达进行了比较。先前的研究表明，ESb-M细胞在细胞表面标志物表达和体外侵袭能力方面与ESb细胞非常相似，但体内研究显示它们的转移能力有所下降。皮下接种ESb-M细胞的同基因动物形成的原发性肿瘤更大，存活时间比同样接种ESb细胞的动物长得多。当使用凝集素大豆凝集素（SBA）时，在一种220 kDa和一种80 kDa细胞表面糖蛋白的糖基化方面观察到明显差异。通过变异特异性单克隆抗体检测到细胞膜蛋白表达的进一步差异。这些特异性配体与65-75 kDa的膜糖蛋白反应，这些膜糖蛋白在ESb-M细胞中比在ESb细胞中更突出。除了这些差异外，这两种细胞系的膜糖蛋白和凝集素染色谱非常相似。细胞表面蛋白中观察到的结构差异是否能解释细胞功能行为（转移行为和贴壁特性）的变化仍有待研究。
