School of Resource & Environment, Anhui Agricultural University, Hefei, Anhui Province 230036, China; Anhui Province Key Laboratory of Integrated Pest Management on Crops, Hefei, China.
School of Plant Protection, Anhui Agricultural University, Hefei, Anhui Province 230036, China; Anhui Province Key Laboratory of Integrated Pest Management on Crops, Hefei, China.
Food Chem. 2022 Mar 30;373(Pt B):131575. doi: 10.1016/j.foodchem.2021.131575. Epub 2021 Nov 9.
Gut microbiota-involved metabolism and intestinal absorption affecting bioaccessibility of triadimefon in strawberry and grape were investigated for the first time by coupling the in vitro digestion model with the Caco-2 cell model. Results showed that the gut microbiota decreased the bioaccessibility of triadimefon in strawberry by 31.00% but failed in grape, probably due to a negative modulation of the colon bacterial activity by dietary components in grapes. A strain of triadimefon-degrading bacteria, Stenotrophomonas maltophilia, was isolated from the gut microbiota and its degradation products were profiled. This study also clarified a significant reduction in transepithelial transport (up to 32.81%) of triadimefon as a result of the barrier effect of gut microbiota. These findings provide new insights on the function of the gut microbiota in pesticide bioaccessibility and highlight the importance of including gut microbiota in pesticide residue risk assessments.
首次通过体外消化模型与 Caco-2 细胞模型相结合，研究了肠道微生物群参与的代谢和肠道吸收对草莓和葡萄中三唑酮生物可及性的影响。结果表明，肠道微生物群使草莓中三唑酮的生物可及性降低了 31.00%，但在葡萄中却没有效果，这可能是由于葡萄中的膳食成分对结肠细菌活性的负调控作用。从肠道微生物群中分离到一株三唑酮降解菌，嗜麦芽寡养单胞菌，并对其降解产物进行了分析。本研究还阐明了由于肠道微生物群的屏障作用，三唑酮的跨上皮转运显著减少（高达 32.81%）。这些发现为肠道微生物群在农药生物可及性中的功能提供了新的见解，并强调了在农药残留风险评估中纳入肠道微生物群的重要性。
