Department of Clinical Pharmacology, College of Medicine, Soonchunhyang University, Cheonan, Republic of Korea.
The Comfort Animal Hospital, Sungbuk-gu, Soonginro-50, Seoul, Republic of Korea.
J Infect Public Health. 2021 Dec;14(12):1864-1869. doi: 10.1016/j.jiph.2021.11.005. Epub 2021 Nov 11.
Wounds remain the most important cause of postoperative mortality and morbidity and generate considerable additional social and healthcare costs. Most wounds are caused by various coliforms, Enterococcus fecalis, Proteus sp., and multidrug resistant Staphylococcus aureus. Wound is one of the leading cause of infections in the under developed and developing countries than developed nations.
A total of 43 samples associated with bacteremia and wound infection were collected. Biochemical characterization and culture characteristics of the drug resistant isolates were studied using MacConkey agar, blood agar and mannitol-salt agar. Antibiotic susceptibility analysis of the isolated strains was performed by disc diffusion method using various antibiotics. Prevalence of dug resistance among bacteria isolated from the wound was studied. The ability of Beta lactamase antibiotic producing bacterial strains were analyzed.
A total of 168 bacterial strains were isolated showed high resistant towards ampicillin (89%), ciprofloxacin (90.8), cefepine (90.5), piperacillin (91.8), oxacillin (92.5), and imipenem (96.5). The isolated bacterial strains showed monobacterial as well as polybacterial growth on the surface of the wound. The isolated bacterial strains revealed 89% sensitivity against norfloxacin and 94.9 sensitivity against vancomycin. About 26% of bacterial strains degraded quinolones, whereas only 14% clinical isolates showed their ability to degrade aminoglycosides. A total of 27% bacteria degraded tetracycline and 51% of isolates degraded carbapenems compounds. Interestingly, E. faecalis was resistant against antibiotics such as, Oxacillin, Nalidic acid, Ofloxacin, Erythromycin, Norfloxacin, Ciprofloxacin, Ampicillin, Tetracycline, Cefepine, Amikacin, Cefurooxime, Vancomycin, Piperacillin, Imipenem and Gentamycin. Moreover, Proteus species was resistant against certain numbers of antibiotics namely, Ampicillin, Piperacillin, Oxacillin, Nalidic acid, Tetracycline, Erythromycin, Cefurooxime, Nitrofurantoin, Vancomycin and Imipenem.
The isolated bacterial strains were resistant against various drugs including vancomycin. Staphylococci, and E. faecalisis strains showed resistance against various classes of antibiotics.
伤口仍然是术后死亡和发病的最重要原因，并产生相当大的额外社会和医疗保健成本。大多数伤口是由各种大肠埃希菌、粪肠球菌、变形杆菌和多药耐药金黄色葡萄球菌引起的。与发达国家相比，伤口是发展中国家和不发达国家感染的主要原因之一。
共收集 43 份与菌血症和伤口感染相关的样本。使用麦康凯琼脂、血琼脂和甘露醇盐琼脂研究耐药分离株的生化特征和培养特征。采用纸片扩散法对分离株进行抗生素敏感性分析，使用各种抗生素。研究了从伤口分离的细菌的耐药性流行情况。分析了产β-内酰胺酶抗生素的细菌菌株的能力。
共分离出 168 株细菌，对氨苄西林（89%）、环丙沙星（90.8%）、头孢吡肟（90.5%）、哌拉西林（91.8%）、苯唑西林（92.5%）和亚胺培南（96.5%）均显示出高度耐药性。从伤口表面分离的细菌菌株表现出单细菌和多细菌生长。分离的细菌菌株对诺氟沙星的敏感性为 89%，对万古霉素的敏感性为 94.9%。约 26%的细菌菌株降解喹诺酮类药物，而只有 14%的临床分离株显示其降解氨基糖苷类药物的能力。总共 27%的细菌降解四环素，51%的分离株降解碳青霉烯类化合物。有趣的是，粪肠球菌对氨苄西林、萘啶酸、氧氟沙星、红霉素、诺氟沙星、环丙沙星、氨苄西林、四环素、头孢吡肟、阿米卡星、头孢呋肟、万古霉素、哌拉西林、亚胺培南和庆大霉素等抗生素均有耐药性。此外，变形杆菌对多种抗生素具有耐药性，包括氨苄西林、哌拉西林、苯唑西林、萘啶酸、四环素、红霉素、头孢呋肟、呋喃妥因、万古霉素和亚胺培南。
分离的细菌菌株对包括万古霉素在内的各种药物均有耐药性。葡萄球菌和粪肠球菌对各种类别的抗生素均有耐药性。
