Key Laboratory of Microbiology, College of Life Sciences, Heilongjiang University, Harbin 150080, China; Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500, China.
College of Basic Medicine, Mudanjiang Medical University, Mudanjiang 157000, China.
Bioresour Technol. 2022 Mar;347:126372. doi: 10.1016/j.biortech.2021.126372. Epub 2021 Nov 18.
In this study, the effects of penicillin G (PENG) on the fate of bacterial communities and β-lactamase antibiotic resistance genes (ARGs) during chicken manure composting were assessed, to illustrate the roles of PENG in ARGs behavior. The results showed that the total absolute abundances of 9 ARGs and 4 mobile genetic elements (MGEs) was significantly increased by PENG (P < 0.05). Dozens of potential hosts for ARGs were predominantly affiliated with Firmicutes, Proteobacteria, and Actinobacteria. Meanwhile, the higher concentration of PENG significantly increased the abundance of luxI and luxS in quorum sensing (QS) (P < 0.05), which enhanced the frequency of inter/intraspecific gene "communication." Redundancy analysis and structural equation modeling further revealed that QS had a strong regulatory role in horizontal gene transfer of ARGs mediated via MGEs. These results provide new insight into the mechanism of ARGs propagation in aerobic composting modified by PENG.
在这项研究中，评估了青霉素 G（PENG）对鸡粪堆肥过程中细菌群落和β-内酰胺类抗生素抗性基因（ARGs）命运的影响，以阐明 PENG 在 ARGs 行为中的作用。结果表明，PENG 显著增加了 9 种 ARGs 和 4 种移动遗传元件（MGEs）的总绝对丰度（P < 0.05）。数十个潜在的 ARGs 宿主主要与厚壁菌门、变形菌门和放线菌门有关。同时，较高浓度的 PENG 显著增加了群体感应（QS）中 luxI 和 luxS 的丰度（P < 0.05），从而增强了种间/种内基因“交流”的频率。冗余分析和结构方程模型进一步表明，QS 对 MGEs 介导的 ARGs 水平基因转移具有很强的调控作用。这些结果为 PENG 修饰的好氧堆肥中 ARGs 传播机制提供了新的见解。
