Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Mol Biol Rep. 2022 Feb;49(2):1361-1367. doi: 10.1007/s11033-021-06966-x. Epub 2021 Nov 21.
Rodentolepis nana (syn. Hymenolepis nana), the most common cyclophyllid tapeworm infecting rodents, is a well-studied gastrointestinal parasite in mice and belongs to the family Hymenolepididae.
The present study focuses on the molecular analysis for the nuclear genes (ITS-1, 18 S, and 28 S rDNA) used for the accurate recognition of the recovered Rodentolepis species.
The annotated partial ITS-1, 18 S, and 28 S rDNA gene regions were deposited in GenBank (gbǀ MW310394.1, gbǀ MW327585.1, and gbǀ MW324479.1, respectively) and further used in the maximum likelihood method (ML) to clarify their genetic relationships at the species level. The interrogation sequence of R. nana was aligned and belonged to the family Hymenolepididae, in the same group as all Hymenolepis species, which were distinct from Cyclophyllidea cestodes, especially species belonging to Anoplocephalidae and Taeniidae. Sequence data support the paraphyly of Hymenolepis species.
The phylogeny supports the availability of the ITS-1, 18 S, and 28 S rDNA genes as reliable genetic markers for evolutionary relationships.
Rodentolepis nana（曾用名 Hymenolepis nana）是感染啮齿动物的最常见的环带绦虫，是研究小鼠胃肠道寄生虫的良好模型，属于膜壳科。
本研究侧重于对核基因（ITS-1、18S 和 28S rDNA）进行分子分析，用于准确识别回收的 Rodentolepis 物种。
注释的部分 ITS-1、18S 和 28S rDNA 基因区域已在 GenBank 中（gb|MW310394.1、gb|MW327585.1 和 gb|MW324479.1）进行了存放，并进一步用于最大似然法（ML），以明确它们在种间水平的遗传关系。R. nana 的查询序列与膜壳科的所有 Hymenolepis 物种相匹配，与 Cyclophyllidea 绦虫不同，特别是与 Anoplocephalidae 和 Taeniidae 物种不同。序列数据支持 Hymenolepis 物种的并系发生。
系统发育支持 ITS-1、18S 和 28S rDNA 基因作为进化关系可靠遗传标记的可用性。
