Ömercioğlu Göktuğ, Akat Fırat, Fıçıcılar Hakan, Billur Deniz, Çalışkan Hasan, Kızıl Şule, Bayram Pınar, Can Belgin, Baştuğ Metin
Department of Physiology, Faculty of Medicine, Ankara University, Ankara, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Ankara University, Ankara, Turkey.
Exp Physiol. 2022 Jan;107(1):42-57. doi: 10.1113/EP089974. Epub 2021 Dec 7.
What is the central question of this study? We evaluated the effects of diabetes and exercise on lipopolysaccharide-induced acute lung injury. By providing a comprehensive analysis of redox status, blood gases and histological parameters, we aimed to contribute to the ongoing debate in the literature. What are the main findings and its importance? We demonstrated the preventive effect of exercise, but diabetes did not alter the severity of acute lung injury.
Acute lung injury (ALI) is a life-threatening respiratory condition. Diabetes (DM) is a metabolic disease characterized by hyperglycaemia. There is an ongoing debate concerning whether there is a protective effect of diabetes in ALI. Exercise is a special type of physical activity that has numerous beneficial effects. The aim of our study was to investigate the effects of diabetes and exercise on the prognosis of ALI. Male Wistar albino rats were divided into two groups (sedentary and exercise). Both groups were divided into four subgroups: Control, ALI, DM, DM+ALI (n = 6 each). Diabetes was induced by injection of streptozotocin (50 mg/kg i.p.). The maximal exercise capacity was determined with the incremental load test. Animals were exercised on a treadmill for 45 min at 70% of maximal exercise capacity, 5 days a week for 12 weeks. Acute lung injury was induced by intratracheal injection of lipopolysaccharide (100 μg/100 g body weight) 24 h before the end of the experiment. We performed arterial blood gas analysis. Redox status was measured in both plasma and lung tissue. Malondialdehyde and 8-hydroxy-2'-deoxyguanosine levels were measured in lung tissue. Lung tissue was evaluated histologically. Acute lung injury caused significant damage in the lung tissue, which was verified histologically, with an increase in oxidative stress parameters. Exercise prevented the lung damage induced by ALI and reduced oxidative stress in the lung tissue. Diabetes did not alter the magnitude of damage done by ALI. Exercise showed a protective effect against DM and ALI in rats. The effect of DM was insignificant for the prognosis of ALI.
本研究的核心问题是什么？我们评估了糖尿病和运动对脂多糖诱导的急性肺损伤的影响。通过对氧化还原状态、血气和组织学参数进行全面分析，我们旨在为文献中正在进行的争论提供参考。主要发现及其重要性是什么？我们证明了运动的预防作用，但糖尿病并未改变急性肺损伤的严重程度。
急性肺损伤（ALI）是一种危及生命的呼吸系统疾病。糖尿病（DM）是一种以高血糖为特征的代谢性疾病。关于糖尿病在ALI中是否具有保护作用，目前仍存在争议。运动是一种具有众多有益作用的特殊身体活动。我们研究的目的是调查糖尿病和运动对ALI预后的影响。将雄性Wistar白化大鼠分为两组（久坐组和运动组）。两组又各分为四个亚组：对照组、ALI组、DM组、DM + ALI组（每组n = 6）。通过腹腔注射链脲佐菌素（50 mg/kg）诱导糖尿病。通过递增负荷试验确定最大运动能力。动物在跑步机上以最大运动能力的70%运动45分钟，每周5天，共12周。在实验结束前24小时，通过气管内注射脂多糖（100 μg/100 g体重）诱导急性肺损伤。我们进行了动脉血气分析。在血浆和肺组织中测量氧化还原状态。在肺组织中测量丙二醛和8-羟基-2'-脱氧鸟苷水平。对肺组织进行组织学评估。急性肺损伤在肺组织中造成了显著损伤，这在组织学上得到了证实，同时氧化应激参数增加。运动预防了ALI诱导的肺损伤，并降低了肺组织中的氧化应激。糖尿病并未改变ALI造成的损伤程度。运动对大鼠的DM和ALI具有保护作用。DM对ALI预后的影响不显著。
