Key Laboratory of Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, College of Bioscience and Resource Environment, Beijing University of Agriculture, Beijing 102206, China.
Key Laboratory of Northern Urban Agriculture of Ministry of Agriculture and Rural Affairs, College of Bioscience and Resource Environment, Beijing University of Agriculture, Beijing 102206, China.
Pestic Biochem Physiol. 2021 Nov;179:104962. doi: 10.1016/j.pestbp.2021.104962. Epub 2021 Sep 10.
Chitin metabolism has long been considered promising targets for development of biorational pesticides. Considering the increasing challenges of controlling the twospotted spider mite, Tetranychus urticae Koch, the roles of chitin deacetylases (CDAs) during molting process and mite development are explored. TuCDA1 and TuCDA2 differ in expression patterns during the development process. Feeding of double-strand RNA (dsRNA) against TuCDA1 or TuCDA2 has lethal effects on the mites. Especially TuCDA2 displays a much stronger phenotype than TuCDA1 (p = 0.0003). The treated mites fail to shed the old cuticle and are trapped within exuviate until they die. The aberrant cuticle structure observed by scanning electronmicroscopy (SEM) and transmission electron microscopy (TEM) may be responsible for the lethal phenotype of TuCDA1 and TuCDA2 knocked down mites. However, treatment with both dsRNA-CDA1 and dsRNA-CDA2 cannot significantly enhance the lethal effects of dsRNA-CDA2, which indicates partially redundant function of TuCDA1 and TuCDA2. TuCDA2 may play a key role during the molting and development process. Chitin-modifying enzyme such as TuCDA2 is potential target of RNA interference through feeding.
几丁质代谢一直被认为是生物合理农药开发的有前途的靶标。考虑到控制二斑叶螨（Tetranychus urticae Koch）的挑战日益增加，探索了几丁质去乙酰化酶（CDA）在蜕皮过程和螨发育中的作用。TuCDA1 和 TuCDA2 在发育过程中的表达模式不同。针对 TuCDA1 或 TuCDA2 的双链 RNA（dsRNA）的饲喂对螨虫具有致死作用。特别是 TuCDA2 比 TuCDA1 表现出更强的表型（p = 0.0003）。处理过的螨虫无法蜕皮，被困在蜕皮中直到死亡。扫描电子显微镜（SEM）和透射电子显微镜（TEM）观察到的异常表皮结构可能是导致 TuCDA1 和 TuCDA2 敲低螨虫致死表型的原因。然而，用 dsRNA-CDA1 和 dsRNA-CDA2 处理不能显著增强 dsRNA-CDA2 的致死作用，这表明 TuCDA1 和 TuCDA2 具有部分冗余功能。TuCDA2 在蜕皮和发育过程中可能起关键作用。几丁质修饰酶如 TuCDA2 可能是通过饲喂进行 RNA 干扰的潜在靶标。
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