Department of Biochemistry, Institute of Chemistry, University of São Paulo, São Paulo, SP 05508, Brazil.
Rutgers Cancer Institute of New Jersey, Newark, NJ 07101, USA.
STAR Protoc. 2021 Nov 8;2(4):100936. doi: 10.1016/j.xpro.2021.100936. eCollection 2021 Dec 17.
This protocol describes the genomic phage (gPhage) display platform, a large-scale antigen and epitope mapping technique. We constructed a gPhage display peptide library of a eukaryotic organism, (causative agent of Chagas disease), to map the antibody response landscape against the parasite. Here, we used an organism with a relatively large but intronless genome, although future applications could include other prevalent or (re)emerging infectious organisms carrying genomes with a limited number of introns. For complete details on the use and execution of this protocol, please refer to Teixeira et al. (2021).
本方案描述了基因组噬菌体（gPhage）展示平台，这是一种大规模的抗原和表位作图技术。我们构建了真核生物（恰加斯病病原体）的 gPhage 展示肽文库，以绘制针对寄生虫的抗体反应图谱。在这里，我们使用了一个相对较大但内含子较少的基因组的生物体，尽管未来的应用可能包括其他流行或（再）出现的携带有限数量内含子的基因组的传染性生物体。有关该方案使用和执行的完整详细信息，请参见 Teixeira 等人。（2021 年）。
