Dahlberg A E, Horodyski F, Keller P
Antimicrob Agents Chemother. 1978 Feb;13(2):331-9. doi: 10.1128/AAC.13.2.331.
Neomycin binds ribosomes and ribosomal ribonucleic acid (rRNA) in vivo and in vitro producing changes detectable by increases in gel electrophoretic mobility. These changes were observed in gels that contain ethylenediaminetetraacetic acid or no added magnesium ion. The progressive increase in gel electrophoretic mobility with increasing antibiotic concentrations suggests that neomycin is binding at multiple sites on RNA. The binding was reversible but sufficiently stable to survive dialysis and electrophoresis. It is proposed that bound neomycin stabilizes the ribosome and RNA structures, restricting the unfolding of the particles during electrophoresis and thus allowing for a more rapid migration in the gel. Gentamicin produced an effect similar to that of neomycin. Paromomycin, differing from neomycin by only one amino group, had considerably less effect on ribosome and rRNA mobilities. The binding of neomycin to rRNA improved the linearity of the plot of log molecular weight versus mobility and thus may be of benefit in providing a more accurate estimation of molecular weights of large RNAs.
新霉素在体内和体外均能与核糖体及核糖体核糖核酸（rRNA）结合，导致凝胶电泳迁移率增加，从而产生可检测到的变化。在含有乙二胺四乙酸或未添加镁离子的凝胶中均观察到了这些变化。随着抗生素浓度的增加，凝胶电泳迁移率逐渐升高，这表明新霉素在RNA的多个位点上发生结合。这种结合是可逆的，但稳定性足以在透析和电泳过程中保持。有人提出，结合的新霉素会稳定核糖体和RNA结构，在电泳过程中限制颗粒的展开，从而使其在凝胶中迁移得更快。庆大霉素产生的效果与新霉素类似。巴龙霉素与新霉素仅相差一个氨基，对核糖体和rRNA迁移率的影响要小得多。新霉素与rRNA的结合改善了对数分子量与迁移率关系图的线性，因此可能有助于更准确地估计大RNA的分子量。
