Polack F, Lowenthal S
LURE, Université de Paris XI, Orsay, France.
Scanning Microsc Suppl. 1987;1:41-6.
In the soft x-ray region, different techniques have been recently developed which can give images of biological objects at an intermediate resolution between optical and electron microscopes. At these wavelengths, very little sample preparation is required because the image contrast is directly dependent upon the chemical composition of the sample itself and on the x-ray wavelength. X-ray microscopy can be extended to absorption microanalysis with a similar resolution, provided that the basic microscope can work through a large range of wavelengths. The photoelectron x-ray microscope that we are developing at LURE satisfies this condition. Its principle is to make a contact x-ray image of the object on a plane photocathode and then, to magnify the secondary electron image, into which it is converted, by means of an emission electron microscope. In the present state of the art, the expectable detection limit, for element maps of 0.1 micron resolution, would be near 1% in concentration.
在软X射线区域,最近已开发出不同的技术,这些技术能够在光学显微镜和电子显微镜之间的中等分辨率下给出生物物体的图像。在这些波长下,所需的样品制备非常少,因为图像对比度直接取决于样品本身的化学成分以及X射线波长。只要基本显微镜能够在很宽的波长范围内工作,X射线显微镜就可以扩展到具有类似分辨率的吸收微分析。我们在法国国家同步辐射实验室(LURE)正在开发的光电子X射线显微镜满足这一条件。其原理是在平面光电阴极上对物体进行接触式X射线成像,然后通过发射电子显微镜放大转换而成的二次电子图像。在目前的技术水平下,对于分辨率为0.1微米的元素图,预期的检测限在浓度方面将接近1%。