基于硝基还原酶 Rv2466c 的硝基呋喃 calanolide-海藻糖探针检测。
Detecting using a nitrofuranyl calanolide-trehalose probe based on nitroreductase Rv2466c.
机构信息
School of Pharmaceutical Sciences, Tsinghua University, Haidian Dist., Beijing 100084, P. R. China.
Tsinghua-Peking Center for Life Sciences, Handian Dist., Beijing 100084, P. R. China.
出版信息
Chem Commun (Camb). 2021 Dec 7;57(97):13174-13177. doi: 10.1039/d1cc05187c.
A new Mtb fluorescent probe, NFC-Tre-5, was reported that could label single cells of Mtb under various stress conditions a unique fluorescence off-on feature by a Rv2466c-mediated reductive mechanism. This probe effectively facilitates the rapid and specific detection of Mtb in the host cell during infection and the detection of Mtb in sputum samples from patients.
一种新的 Mtb 荧光探针，NFC-Tre-5，被报道能够在各种应激条件下标记 Mtb 的单个细胞，通过 Rv2466c 介导的还原机制具有独特的荧光关闭-开启特性。该探针有效地促进了在感染过程中宿主细胞中 Mtb 的快速和特异性检测，以及从患者的痰样本中检测 Mtb。
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