致幻吲哚烷基胺及其体内代谢产物的气相色谱-质谱表征

Gas chromatographic-mass spectroscopic characterisation of the psychotomimetic indolealkylamines and their in vivo metabolites.

作者信息

Sitaram B R, Lockett L, McLeish M, Hayasaka Y, Blackman G L, McLeod W R

机构信息

School of Pharmaceutical Chemistry, Victorian College of Pharmacy, Parkville, Victoria, Australia.

出版信息

J Chromatogr. 1987 Nov 27;422:13-23. doi: 10.1016/0378-4347(87)80435-8.

DOI:10.1016/0378-4347(87)80435-8

PMID:3481368

Abstract

The use of liquid chromatography with on-line fluorescence detection has formed the basis for the separation, characterisation and quantitation of a number of metabolites of the psychotomimetic indolealkylamines N,N-dimethyltryptamine and 5-methoxy-N,N-dimethyltryptamine formed both in vitro and in vivo. Verification of the identity of metabolites has previously been facilitated by the combined use of a number of analytical techniques including multidimensional liquid chromatography and stop-flow spectroscopic analysis. We now describe the combination of liquid chromatography with gas chromatography-mass spectrometry for the unequivocal verification of a number of structurally characteristic metabolites of the psychotomimetic indolealkylamines.

摘要

采用液相色谱-在线荧光检测法，已成为对致幻吲哚烷基胺N,N-二甲基色胺和5-甲氧基-N,N-二甲基色胺在体外和体内形成的多种代谢产物进行分离、表征和定量分析的基础。此前，通过联合使用多种分析技术，包括多维液相色谱和停流光谱分析，有助于对代谢产物的身份进行验证。我们现在描述液相色谱与气相色谱-质谱联用，用于明确验证致幻吲哚烷基胺的多种具有结构特征的代谢产物。