Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials, Ministry of Education, Shandong University, Jinan, China; Shenzhen Research Institute of Shandong University, Shenzhen, China.
Department of Clinical Laboratory, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan, China.
Talanta. 2022 Feb 1;238(Pt 2):123077. doi: 10.1016/j.talanta.2021.123077. Epub 2021 Nov 17.
As a class of newly identified biomarkers, miRNAs show enormous potential in cancer diagnosis. The sensitive detection of abnormal miRNAs concentration to realize early diagnosis of malignant tumors is a frontier in the field of biosensing. In this work, a photoelectrochemical (PEC) biosensor based on MoS@TiC nanohybrid was fabricated for the ultrasensitive detection of miRNAs. The hybridization of TiC with excellent electron transfer capability significantly enhances the photocurrent response of the PEC biosensor. Moreover, the electrodeposition of Au nanoparticles on the surface of MoS@TiC nanohybrid further enhances the photocurrent. The detection performance of the PEC biosensor has been tested using colorectal cancer-related exosomal miRNA (miR-92a-3p) as the target. The PEC biosensor shows a broad linear detection ranged from 1 fM to 100 nM and a calculated detection limit of 0.27 fM. In terms of selectivity, the PEC biosensor can distinguish miR-92a-3p from mismatched sequences. The 16 continuous radiation source on-off cycles test indicates the high stability of the PEC biosensor. Furthermore, the accurate detection of exosomal miR-92a-3p concentrations of patients and healthy controls demonstrates the clinical feasibility of the PEC biosensor. Based on these outcomes, the PEC biosensor exhibits the prospect of realizing the ultrasensitive point-of-care detection of miRNAs.
作为一类新鉴定的生物标志物，miRNA 在癌症诊断中具有巨大的潜力。敏感检测异常 miRNA 浓度以实现恶性肿瘤的早期诊断是生物传感领域的一个前沿。在这项工作中，构建了基于 MoS@TiC 纳米杂化材料的光电化学（PEC）生物传感器，用于超灵敏检测 miRNA。具有优异电子传递能力的 TiC 的杂交显著增强了 PEC 生物传感器的光电流响应。此外，在 MoS@TiC 纳米杂化材料表面电沉积 Au 纳米颗粒进一步增强了光电流。使用结直肠癌相关外泌体 miRNA（miR-92a-3p）作为靶标测试了 PEC 生物传感器的检测性能。PEC 生物传感器具有从 1 fM 到 100 nM 的宽线性检测范围和计算出的 0.27 fM 的检测限。在选择性方面，PEC 生物传感器可以区分 miR-92a-3p 和错配序列。16 个连续辐射源开-关循环测试表明 PEC 生物传感器具有高稳定性。此外，对患者和健康对照者外泌体 miR-92a-3p 浓度的准确检测证明了 PEC 生物传感器的临床可行性。基于这些结果，PEC 生物传感器显示了实现 miRNA 超灵敏即时检测的前景。
