Schrama Denise, Raposo de Magalhães Cláudia, Cerqueira Marco, Carrilho Raquel, Farinha Ana Paula, Rosa da Costa Ana M, Gonçalves Amparo, Kuehn Annette, Revets Dominique, Planchon Sébastien, Engrola Sofia, Rodrigues Pedro M
Universidade do Algarve, Campus de Gambelas, Faro, Portugal; CCMAR, Centre of Marine Sciences, Universidade do Algarve, Campus de Gambelas, Faro, Portugal.
CCMAR, Centre of Marine Sciences, Universidade do Algarve, Campus de Gambelas, Faro, Portugal.
Comp Biochem Physiol Part D Genomics Proteomics. 2022 Mar;41:100941. doi: 10.1016/j.cbd.2021.100941. Epub 2021 Nov 16.
The relatively easy access to fish worldwide, alongside the increase of aquaculture production contributes to increased fish consumption which result in higher prevalence of respective allergies. Allergies to fish constitute a significant concern worldwide. β-parvalbumin is the main elicitor for IgE-mediated reactions. Creatine, involved in the muscle energy metabolism, and ethylenediamine tetraacetic acid (EDTA), a calcium chelator, are potential molecules to modulate parvalbumin. The purpose of this study was to test creatine (2, 5 and 8%) and EDTA (1.5, 3 and 4.5%) supplementation in fish diets to modulate β-parvalbumin expression and structure and its allergenicity in farmed European seabass (Dicentrarchus labrax) while assessing its effects on the end-product quality. Fish welfare and muscle quality parameters were evaluated by plasma metabolites, rigor mortis, muscle pH and sensory and texture analysis. Proteomics was used to assess alterations in muscle proteome profile and metabolic fingerprinting by Fourier transform infrared spectroscopy was used to assess the liver metabolic profile. In addition, IgE-reactivity to parvalbumin was analysed using fish allergic patient sera. Metabolic fingerprinting of liver tissue revealed no major alterations in infrared spectra with creatine supplementation, while with EDTA, only absorption bands characteristic of lipids were altered. Comparative proteomics showed up regulation of (tropo) myosin and phosphoglycerate mutase 2 with Creatine supplementation. In the case of EDTA proteomics showed up regulation of proteins involved in cellular and ion homeostasis. Allergenicity seems not to be modulated with creatine or EDTA supplementation as no decreased expression levels were found and IgE-binding reactivity showed no quantitative differences.
全球范围内鱼类获取相对容易，加之水产养殖产量增加，导致鱼类消费量上升，进而使相应过敏症的患病率更高。鱼类过敏是全球范围内的一个重大问题。β-副肌球蛋白是IgE介导反应的主要引发剂。肌酸参与肌肉能量代谢，而乙二胺四乙酸（EDTA）是一种钙螯合剂，它们都是调节副肌球蛋白的潜在分子。本研究的目的是测试在养殖的欧洲海鲈（Dicentrarchus labrax）的鱼饲料中添加肌酸（2%、5%和8%）和EDTA（1.5%、3%和4.5%），以调节β-副肌球蛋白的表达、结构及其致敏性，同时评估其对最终产品质量的影响。通过血浆代谢物、尸僵、肌肉pH值以及感官和质地分析来评估鱼类福利和肌肉质量参数。蛋白质组学用于评估肌肉蛋白质组图谱的变化，傅里叶变换红外光谱法用于评估肝脏代谢图谱的代谢指纹分析。此外，使用鱼类过敏患者血清分析对副肌球蛋白的IgE反应性。肝脏组织的代谢指纹分析显示，添加肌酸后红外光谱没有重大变化，而添加EDTA时，仅脂质特征吸收带发生了改变。比较蛋白质组学表明，添加肌酸后（原肌）肌球蛋白和磷酸甘油酸变位酶2上调。在EDTA的情况下，蛋白质组学显示参与细胞和离子稳态的蛋白质上调。添加肌酸或EDTA似乎并未调节致敏性，因为未发现表达水平降低，且IgE结合反应性没有定量差异。
